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Literature summary for 3.6.1.5 extracted from

  • Sevigny, J.; Robson, S.C.; Waelkens, E.; Csizmadia, E.; Smith, R.N.; Lemmens, R.
    Identification and characterization of a novel hepatic canalicular ATP diphosphohydrolase (2000), J. Biol. Chem., 275, 5640-5647.
    View publication on PubMed
Search for more literature for 3.6.1.5

Inhibitors

Inhibitors Comment Organism Structure
5'-p-fluorosulfonylbenzoyladenosine 50% inhibition at 2 mM Sus scrofa
EGTA
-
 Sus scrofa
additional information no inhibition by NaN3 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 ATP
-
 Sus scrofa
0.1
-
 ADP
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane ectonucleotidase/ectoenzyme Sus scrofa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ preferred to Mg2+ Sus scrofa
Mg2+ either Mg2+ or Ca2+ required for activity Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 highly glycosylated protein, N-linked oligosaccarides, immunoaffinity techniques, SDS-PAGE Sus scrofa
78000
-
 immunoaffinity techniques, SDS-PAGE, ATPDase/cd39 Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Sus scrofa
-
 AMP + phosphate
-
 ?
ATP + 2 H2O Sus scrofa
-
 AMP + 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 pig
-
Sus scrofa
-
 enzyme belongs to E-type ATPase/NTPDase family
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-linked oligosaccarides Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
purified from liver Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
duodenum
-
 Sus scrofa
-
kidney ATPDase/cd39 detected by immunoblotting in vascular endothelium and smooth muscles (blood vessel walls) Sus scrofa
-
kidney ATPDase detected by immunoblotting in luminal side of the ductular epithelium Sus scrofa
-
liver ATPDase/cd39 detected by immunoblotting in vascular endothelium and smooth muscles (blood vessel walls) Sus scrofa
-
liver ATPDase detected by immunoblotting in bile canaliculi of hepatocytes Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.4
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Sus scrofa AMP + phosphate
-
 ?
ATP + 2 H2O
-
 Sus scrofa AMP + 2 phosphate
-
 ?
CDP + H2O
-
 Sus scrofa CMP + phosphate
-
 ?
CTP + 2 H2O
-
 Sus scrofa CMP + 2 phosphate
-
 ?
GDP + H2O
-
 Sus scrofa GMP + phosphate
-
 ?
GTP + 2 H2O
-
 Sus scrofa GMP + 2 phosphate
-
 ?
IDP + H2O
-
 Sus scrofa IMP + phosphate
-
 ?
ITP + 2 H2O
-
 Sus scrofa IMP + 2 phosphate
-
 ?
additional information neglible hydrolysis of AMP Sus scrofa ?
-
 ?
TDP + H2O
-
 Sus scrofa TMP + phosphate
-
 ?
TTP + 2 H2O
-
 Sus scrofa TMP + 2 phosphate
-
 ?
UDP + H2O
-
 Sus scrofa UMP + phosphate
-
 ?
UTP + 2 H2O
-
 Sus scrofa UMP + 2 phosphate
-
 ?

Synonyms

Synonyms Comment Organism
nucleoside triphosphate diphosphohydrolase-1, NDPDase 1
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 8
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 50% activity at pH 6, no activity at pH 9.5 Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
 5'-p-fluorosulfonylbenzoyladenosine
-
 Sus scrofa