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Literature summary for 3.6.1.45 extracted from
- Clostridium difficile toxins A and B are cation-dependent UDP-glucose hydrolases with differing catalytic activities (1998), J. Biol. Chem., 273, 16021-16026.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Clostridioides difficile |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0894 | - |
UDP-glucose | with MnCl2 | Clostridioides difficile | |
| 0.1215 | - |
UDP-glucose | isozyme toxin A, with MnCl2 | Clostridioides difficile | |
| 0.1423 | - |
UDP-glucose | isozyme toxin A, with MgCl2 and 150 mM KCl | Clostridioides difficile | |
| 0.1435 | - |
UDP-glucose | isozyme toxin A, with 50 mM KCl | Clostridioides difficile | |
| 0.1574 | - |
UDP-glucose | isozyme toxin B, with 50 mM KCl | Clostridioides difficile | |
| 0.1638 | - |
UDP-glucose | isozyme toxin A, with 500 mM KCl | Clostridioides difficile | |
| 0.1735 | - |
UDP-glucose | isozyme toxin B, with 500 mM KCl | Clostridioides difficile | |
| 0.1755 | - |
UDP-glucose | isozyme toxin B, with MgCl2 and 150 mM KCl | Clostridioides difficile | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | absolute requirement for K+ | Clostridioides difficile | |
| Mg2+ | divalent metal ion required, significantly lower Km in presence of Mn2+ as compared with Mg2+ | Clostridioides difficile | |
| Mn2+ | divalent metal ion required, significantly lower Km in presence of Mn2+ as compared with Mg2+ | Clostridioides difficile | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridioides difficile | - |
2 different enzyme forms: toxin A and toxin B | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-glucose | - |
Clostridioides difficile | UMP + glucose 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| toxin A | - |
Clostridioides difficile |
| toxin B | - |
Clostridioides difficile |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 40 | - |
Clostridioides difficile |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8 | - |
Clostridioides difficile |
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Release 2023.1 (January 2023)
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