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Literature summary for 3.6.1.45 extracted from

  • Glaser, L.; Melo, A.; Paul, R.
    Uridine diphosphate sugar hydrolase. Purification of enzyme and protein inhibitor (1967), J. Biol. Chem., 242, 1944-1954.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP-D-glucose competitive Escherichia coli
CDP-D-glucose competitive Escherichia coli
GDP-D-glucose competitive Escherichia coli
Inhibitor from E. coli
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
0.002
-
UDP-D-glucose with 5 mM CoSO4, pH 8.0, 37 °C Escherichia coli
0.02
-
UDP-D-glucose with 5 mM CoSO4, pH 8.0, 37 °C Escherichia coli
0.11
-
GDP-D-glucose
-
Escherichia coli
0.2
-
UDP-D-glucose with 5 mM MgCl2, pH 8.0, 25 °C Escherichia coli
0.33
-
CDP-D-glucose
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective Escherichia coli
Mg2+ absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective Escherichia coli
Mn2+ absolute requirement for a divalent cation, at pH 8.0 in Tris-HCl buffer the requirement can be satisfied in order of decreasing affinity by Co2+, Mn2+, Mg2+. At pH 5.1 in acetate buffer only Co2+ and Mn2+ are effective Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
ATCC 12793
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.58
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-D-glucose at 5% of the activity with UDP-glucose Escherichia coli CMP + glucose 1-phosphate
-
?
GDP-glucose at 5% of the activity with UDP-glucose Escherichia coli GMP + glucose 1-phosphate
-
?
UDP-D-glucose
-
Escherichia coli UMP + glucose 1-phosphate
-
?
UDP-galactose
-
Escherichia coli UMP + galactose 1-phosphate
-
?
UDP-N-acetyl-D-galactosamine
-
Escherichia coli UMP + N-acetyl-D-galactosamine 1-phosphate
-
?
UDP-N-acetyl-D-glucosamine
-
Escherichia coli UMP + N-acetyl-D-glucosamine 1-phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Co2+, Mn2+ or Mg2+ protect against heat inactivation Escherichia coli