BRENDA - Enzyme Database
show all sequences of 3.6.1.43

The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae

Fernandez, F.; Rush, J.S.; Toke, D.A.; Han, G.S.; Quinn, J.E.; Carman, G.M.; Choi, J.Y.; Voelker, D.R.; Aebi, M.; Waechter, C.J.; J. Biol. Chem. 276, 41455-41464 (2001)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression in Saccharomyces cerevisiae and Sf-9 insect cells
Saccharomyces cerevisiae
General Stability
General Stability
Organism
the enzyme is labile to Triton X-100 when expressed in Sf-9 cells
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
dolichyl diphosphate
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
Saccharomyces cerevisiae
0.048
-
dolichyl phosphate
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
transmembrane protein with a luminally oriented active site
Saccharomyces cerevisiae
5783
-
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dolichyl diphosphate + H2O
-
656027
Saccharomyces cerevisiae
dolichyl phosphate + phosphate
-
-
-
?
dolichyl phosphate + H2O
-
656027
Saccharomyces cerevisiae
dolichol + phosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Dol-P-P phosphatase
-
Saccharomyces cerevisiae
dolichyl pyrophosphate phosphatase
-
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
in the presence of octyl glucoside
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Saccharomyces cerevisiae and Sf-9 insect cells
Saccharomyces cerevisiae
General Stability (protein specific)
General Stability
Organism
the enzyme is labile to Triton X-100 when expressed in Sf-9 cells
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
dolichyl diphosphate
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
Saccharomyces cerevisiae
0.048
-
dolichyl phosphate
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
transmembrane protein with a luminally oriented active site
Saccharomyces cerevisiae
5783
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dolichyl diphosphate + H2O
-
656027
Saccharomyces cerevisiae
dolichyl phosphate + phosphate
-
-
-
?
dolichyl phosphate + H2O
-
656027
Saccharomyces cerevisiae
dolichol + phosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
in the presence of octyl glucoside
Saccharomyces cerevisiae
Other publictions for EC 3.6.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711954
Manabe
Membrane-bound acid pyrophosph ...
Sulfurisphaera tokodaii
Extremophiles
13
859-865
2009
-
-
1
-
-
-
1
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
5
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
1
1
1
1
-
-
687781
Rush
Recycling of dolichyl monophos ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
4087-4093
2008
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
656097
Rush
Identification and characteriz ...
Mus musculus
J. Biol. Chem.
277
45226-45234
2002
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
2
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656027
Fernandez
The CWH8 gene encodes a dolich ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
41455-41464
2001
-
-
1
-
-
1
-
2
2
-
-
-
-
3
-
-
-
-
-
1
-
-
2
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
2
2
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134921
Bhat
Developmental changes in enzym ...
Rattus norvegicus
J. Neurochem.
56
339-344
1991
-
-
-
-
-
-
-
2
-
-
-
1
-
2
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
1
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289082
Shabalin
-
Intracellular distribution and ...
Saccharomyces cerevisiae
Biochemistry (Moscow)
56
2032-2041
1991
1
-
-
-
-
-
2
-
3
-
-
1
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289080
Scher
Dolichyl pyrophosphate phospha ...
Bos taurus
Methods Enzymol.
111
547-553
1985
3
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
289079
Scher
Brain dolichyl pyrophosphate p ...
Bos taurus
J. Biol. Chem.
259
14580-14585
1984
4
-
-
-
-
-
9
1
3
2
-
1
-
1
-
-
-
-
-
1
1
2
2
-
-
-
-
1
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
9
-
1
3
2
-
1
-
-
-
-
-
1
1
2
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
134917
Belocopitow
Dolichyl-phosphate phosphatase ...
Rattus norvegicus
Eur. J. Biochem.
125
167-173
1982
1
-
-
-
-
-
7
-
1
1
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
-
-
1
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289076
Applekvist
-
Dolichol (C55) mono-and pyroph ...
Rattus norvegicus
Biosci. Rep.
1
619-626
1981
1
-
-
-
-
-
3
-
6
1
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
6
1
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
289077
Boscoboinik
Assay of dolichol monophosphat ...
Rattus norvegicus
Anal. Biochem.
114
42-45
1981
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
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1
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-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134908
Wedgwood
Enzymatic activities in cultur ...
Homo sapiens
J. Biol. Chem.
255
1120-1123
1980
1
-
-
-
-
-
1
-
1
1
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
-
-
-
1
-
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2
-
-
-
-
-
1
1
-
-
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-