BRENDA - Enzyme Database
show all sequences of 3.6.1.43

Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate

Wedgwood, J.F.; Strominger, J.L.; J. Biol. Chem. 255, 1120-1123 (1980)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
-
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
bacitracin
no inhibition when preincubating with EDTA
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Homo sapiens
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ions required
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dolichyl diphosphate + H2O
Homo sapiens
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
lymphocyte
RPMI 4265
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dolichyl diphosphate + H2O
-
134908
Homo sapiens
dolichyl phosphate + phosphate
-
134908
Homo sapiens
?
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
134908
Homo sapiens
dolichyl phosphate + phosphate
-
134908
Homo sapiens
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
95% and 90% of activity, broad pH-range
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
bacitracin
no inhibition when preincubating with EDTA
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Homo sapiens
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ions required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dolichyl diphosphate + H2O
Homo sapiens
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
dolichyl phosphate + phosphate
-
Homo sapiens
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
lymphocyte
RPMI 4265
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dolichyl diphosphate + H2O
-
134908
Homo sapiens
dolichyl phosphate + phosphate
-
134908
Homo sapiens
?
dolichyl diphosphate + H2O
involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
134908
Homo sapiens
dolichyl phosphate + phosphate
-
134908
Homo sapiens
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
95% and 90% of activity, broad pH-range
Homo sapiens
Other publictions for EC 3.6.1.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711954
Manabe
Membrane-bound acid pyrophosph ...
Sulfurisphaera tokodaii
Extremophiles
13
859-865
2009
-
-
1
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1
-
1
-
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5
-
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5
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1
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1
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1
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1
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-
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-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
1
1
1
1
-
-
687781
Rush
Recycling of dolichyl monophos ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
4087-4093
2008
-
-
-
-
-
-
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1
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1
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2
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2
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1
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1
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1
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-
2
-
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-
1
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-
-
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-
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656097
Rush
Identification and characteriz ...
Mus musculus
J. Biol. Chem.
277
45226-45234
2002
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1
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1
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3
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1
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1
2
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2
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656027
Fernandez
The CWH8 gene encodes a dolich ...
Saccharomyces cerevisiae
J. Biol. Chem.
276
41455-41464
2001
-
-
1
-
-
1
-
2
2
-
-
-
-
3
-
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-
1
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2
-
-
-
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1
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1
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1
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2
2
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1
-
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2
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1
-
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-
-
-
-
-
-
-
134921
Bhat
Developmental changes in enzym ...
Rattus norvegicus
J. Neurochem.
56
339-344
1991
-
-
-
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2
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1
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2
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1
3
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2
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1
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1
3
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2
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289082
Shabalin
-
Intracellular distribution and ...
Saccharomyces cerevisiae
Biochemistry (Moscow)
56
2032-2041
1991
1
-
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2
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3
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1
-
1
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1
2
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1
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2
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3
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1
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1
2
-
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-
-
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-
-
-
-
-
289080
Scher
Dolichyl pyrophosphate phospha ...
Bos taurus
Methods Enzymol.
111
547-553
1985
3
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
3
-
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-
-
-
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-
3
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1
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1
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-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
289079
Scher
Brain dolichyl pyrophosphate p ...
Bos taurus
J. Biol. Chem.
259
14580-14585
1984
4
-
-
-
-
-
9
1
3
2
-
1
-
1
-
-
-
-
-
1
1
2
2
-
-
-
1
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
9
-
1
3
2
-
1
-
-
-
-
-
1
1
2
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
134917
Belocopitow
Dolichyl-phosphate phosphatase ...
Rattus norvegicus
Eur. J. Biochem.
125
167-173
1982
1
-
-
-
-
-
7
-
1
1
-
1
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1
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1
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2
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1
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1
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1
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7
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1
1
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1
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1
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2
-
1
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1
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-
-
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-
-
-
289076
Applekvist
-
Dolichol (C55) mono-and pyroph ...
Rattus norvegicus
Biosci. Rep.
1
619-626
1981
1
-
-
-
-
-
3
-
6
1
-
1
-
1
-
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1
-
-
1
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2
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-
1
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1
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3
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6
1
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1
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1
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1
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2
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1
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-
-
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289077
Boscoboinik
Assay of dolichol monophosphat ...
Rattus norvegicus
Anal. Biochem.
114
42-45
1981
-
-
-
-
-
-
-
-
-
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1
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1
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1
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2
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1
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1
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2
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-
-
-
-
-
-
134908
Wedgwood
Enzymatic activities in cultur ...
Homo sapiens
J. Biol. Chem.
255
1120-1123
1980
1
-
-
-
-
-
1
-
1
1
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
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-
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1
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-
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1
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1
1
-
1
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-
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1
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2
-
-
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-
-
1
1
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