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Literature summary for 3.6.1.42 extracted from

  • Massalski, C.; Bloch, J.; Zebisch, M.; Steinebrunner, I.
    The biochemical properties of the Arabidopsis ecto-nucleoside triphosphate diphosphohydrolase AtAPY1 contradict a direct role in purinergic signaling (2015), PLoS ONE, 10, e0115832 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin 5fold increase of activity at 0.001 mM Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293 cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
azide 42% inhibition at 10 mM Arabidopsis thaliana
Cu2+
-
Arabidopsis thaliana
EDTA
-
Arabidopsis thaliana
fluoride 27% inhibition at 10 mM Arabidopsis thaliana
additional information not inhibited by NGXT191 and dithiothreitol Arabidopsis thaliana
vanadate 54% inhibition at 1 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0597
-
GDP at pH 6.5 and 37°C Arabidopsis thaliana
0.0744
-
UDP at pH 6.5 and 37°C Arabidopsis thaliana
0.166
-
IDP at pH 6.5 and 37°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
Arabidopsis thaliana 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ strongly activates the enzyme Arabidopsis thaliana
Mg2+ Mg2+ strongly activates the enzyme. 1 mM is used in assay conditions Arabidopsis thaliana
Mn2+ Mn2+ strongly activates the enzyme Arabidopsis thaliana
Ni2+ Ni2+ has no effect on the enzyme activity Arabidopsis thaliana
Zn2+ Zn2+ slightly activates the enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Arabidopsis thaliana
-
GMP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Wassilewskija
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.33
-
unpurified enzyme, at pH 6.5 and 37°C Arabidopsis thaliana
21.7
-
after 70fold purification, at pH 6.5 and 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + H2O
-
Arabidopsis thaliana GMP + phosphate
-
?
GDP + H2O best substrate Arabidopsis thaliana GMP + phosphate
-
?
IDP + H2O
-
Arabidopsis thaliana IMP + phosphate
-
?
additional information ATP, ADP, CTP, CDP, GTP, ITO, TTP, UTP and TDP are no substrates Arabidopsis thaliana ?
-
-
UDP + H2O
-
Arabidopsis thaliana UMP + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 52000, His6-tagged enzyme, calculated from amino acid sequence Arabidopsis thaliana
? x * 61000, His6-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
APY1
-
Arabidopsis thaliana
E-NTPDase
-
Arabidopsis thaliana
ecto-nucleoside triphosphate diphosphohydrolase
-
Arabidopsis thaliana
GDPase
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 the enzyme shows the highest and same level of activity from pH 5.5 to 9.5. There is a significant drop in activity at pH 5.0 and the activity decreases further with increasing acidity. At pH 4.0 the activity is down to 3% of the highest activity observed Arabidopsis thaliana