Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | 5fold increase of activity at 0.001 mM | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in HEK-293 cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 42% inhibition at 10 mM | Arabidopsis thaliana | |
Cu2+ | - |
Arabidopsis thaliana | |
EDTA | - |
Arabidopsis thaliana | |
fluoride | 27% inhibition at 10 mM | Arabidopsis thaliana | |
additional information | not inhibited by NGXT191 and dithiothreitol | Arabidopsis thaliana | |
vanadate | 54% inhibition at 1 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0597 | - |
GDP | at pH 6.5 and 37°C | Arabidopsis thaliana | |
0.0744 | - |
UDP | at pH 6.5 and 37°C | Arabidopsis thaliana | |
0.166 | - |
IDP | at pH 6.5 and 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Arabidopsis thaliana | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ strongly activates the enzyme | Arabidopsis thaliana | |
Mg2+ | Mg2+ strongly activates the enzyme. 1 mM is used in assay conditions | Arabidopsis thaliana | |
Mn2+ | Mn2+ strongly activates the enzyme | Arabidopsis thaliana | |
Ni2+ | Ni2+ has no effect on the enzyme activity | Arabidopsis thaliana | |
Zn2+ | Zn2+ slightly activates the enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | Arabidopsis thaliana | - |
GMP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Wassilewskija | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.33 | - |
unpurified enzyme, at pH 6.5 and 37°C | Arabidopsis thaliana |
21.7 | - |
after 70fold purification, at pH 6.5 and 37°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | - |
Arabidopsis thaliana | GMP + phosphate | - |
? | |
GDP + H2O | best substrate | Arabidopsis thaliana | GMP + phosphate | - |
? | |
IDP + H2O | - |
Arabidopsis thaliana | IMP + phosphate | - |
? | |
additional information | ATP, ADP, CTP, CDP, GTP, ITO, TTP, UTP and TDP are no substrates | Arabidopsis thaliana | ? | - |
- |
|
UDP + H2O | - |
Arabidopsis thaliana | UMP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, His6-tagged enzyme, calculated from amino acid sequence | Arabidopsis thaliana |
? | x * 61000, His6-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
APY1 | - |
Arabidopsis thaliana |
E-NTPDase | - |
Arabidopsis thaliana |
ecto-nucleoside triphosphate diphosphohydrolase | - |
Arabidopsis thaliana |
GDPase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | the enzyme shows the highest and same level of activity from pH 5.5 to 9.5. There is a significant drop in activity at pH 5.0 and the activity decreases further with increasing acidity. At pH 4.0 the activity is down to 3% of the highest activity observed | Arabidopsis thaliana |