Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional complementation of a Saccharomyces GDPase null mutant | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | disruption of gene gda1 leads to a mutant with 90% reduced GDP hydrolysis activity compared to the wild-type, the mutant also shows severely impaired O-mannosylation and reduced cell wall phosphate content and other phenotypes, but the formation of hyphae is uneffected, overview | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Candida albicans | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Candida albicans | |
Mn2+ | stimulates, preferred metal ion | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | Candida albicans | GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen | GMP + phosphate | - |
? | |
additional information | Candida albicans | physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q8TGH6 | gene gda1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Candida albicans | AMP + phosphate | - |
? | |
GDP + H2O | preferred substrate | Candida albicans | GMP + phosphate | - |
? | |
GDP + H2O | GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen | Candida albicans | GMP + phosphate | - |
? | |
additional information | physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview | Candida albicans | ? | - |
? | |
UDP + H2O | - |
Candida albicans | UMP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | enzyme contains a unique sequence in the stem region predicted with high probability to adopt a coil-coil conformation that favors dimerization | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
Gdap1 | - |
Candida albicans |
GDPase | - |
Candida albicans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Candida albicans |