Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.42 extracted from

  • Yanagisawa, K.; Resnick, D.; Abeijon, C.; Robbins, P.W.; Hirschberg, C.B.
    A guanosine diphosphatase enriched in Golgi vesicles of Saccharomyces cerevisiae. Purification and characterization (1990), J. Biol. Chem., 265, 19351-19355.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
GDP
-
Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi lumen
-
Saccharomyces cerevisiae 5796
-
Golgi lumen
-
Schizosaccharomyces pombe 5796
-
Golgi membrane
-
Saccharomyces cerevisiae 139
-
Golgi membrane
-
Schizosaccharomyces pombe 139
-
Golgi vesicle Golgi-like vesicles Saccharomyces cerevisiae
-
-
Golgi vesicle Golgi-like vesicles Schizosaccharomyces pombe
-
-
membrane
-
Saccharomyces cerevisiae 16020
-
membrane
-
Schizosaccharomyces pombe 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for maximal activity Saccharomyces cerevisiae
divalent cations required Saccharomyces cerevisiae
Fe2+ less than 10% activation Saccharomyces cerevisiae
Mg2+ activation Saccharomyces cerevisiae
Mn2+ activation, enzyme with Mn2+ is 60-95% as active as compared with Ca2+ Saccharomyces cerevisiae
Mn2+ in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ Saccharomyces cerevisiae
additional information
-
Saccharomyces cerevisiae
Ni2+ less than 10% activation Saccharomyces cerevisiae
Zn2+ less than 10% activation Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
deglycosylated GDPase, result of proteolytic cleavage of the 55000 Da enzyme during purification, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Saccharomyces cerevisiae GDP is a reaction product following outer-chain mannosylation of luminal proteins and inhibitor of mannosyltransferases. It is hypothesized that GMP then returns to the cytosol. GDPase presumably serves as an essential function in processing of yeast glycoproteins 5'-GMP + phosphate
-
?
GDP + H2O Saccharomyces cerevisiae GDP is known to be an inhibitor of mannosylation reactions in the Golgi lumen, removal of GDP by conversion to GMP, the latter not being an inhibitor of these reactions, would allow mannosylation to proceed 5'-GMP + phosphate
-
?
additional information Saccharomyces cerevisiae role in mannosylation ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Schizosaccharomyces pombe
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae
partial Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3080
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + H2O best substrate Saccharomyces cerevisiae 5'-GMP + phosphate
-
?
GDP + H2O best substrate Schizosaccharomyces pombe 5'-GMP + phosphate
-
?
GDP + H2O highly specific for GDP Saccharomyces cerevisiae 5'-GMP + phosphate
-
?
GDP + H2O GDP is a reaction product following outer-chain mannosylation of luminal proteins and inhibitor of mannosyltransferases. It is hypothesized that GMP then returns to the cytosol. GDPase presumably serves as an essential function in processing of yeast glycoproteins Saccharomyces cerevisiae 5'-GMP + phosphate
-
?
GDP + H2O GDP is known to be an inhibitor of mannosylation reactions in the Golgi lumen, removal of GDP by conversion to GMP, the latter not being an inhibitor of these reactions, would allow mannosylation to proceed Saccharomyces cerevisiae 5'-GMP + phosphate
-
?
additional information substrate specificity Saccharomyces cerevisiae ?
-
?
additional information substrate specificity Schizosaccharomyces pombe ?
-
?
additional information less than 2% of maximum activity with other nucleoside di-, mono-, and triphosphates Saccharomyces cerevisiae ?
-
?
additional information less than 2% of maximum activity with other nucleoside di-, mono-, and triphosphates Schizosaccharomyces pombe ?
-
?
additional information role in mannosylation Saccharomyces cerevisiae ?
-
?
UDP + H2O about 10% as active with UDP compared to GDP Saccharomyces cerevisiae UMP + phosphate
-
?
UDP + H2O about 10% as active with UDP compared to GDP Schizosaccharomyces pombe UMP + phosphate
-
?
UDP + H2O in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ Saccharomyces cerevisiae UMP + phosphate
-
?
UDP + H2O in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ Schizosaccharomyces pombe UMP + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3830
-
GDP
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 8.2 broad pH optimum Saccharomyces cerevisiae