KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
GDP | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi lumen | - |
Saccharomyces cerevisiae | 5796 | - |
Golgi lumen | - |
Schizosaccharomyces pombe | 5796 | - |
Golgi membrane | - |
Saccharomyces cerevisiae | 139 | - |
Golgi membrane | - |
Schizosaccharomyces pombe | 139 | - |
Golgi vesicle | Golgi-like vesicles | Saccharomyces cerevisiae | - |
- |
Golgi vesicle | Golgi-like vesicles | Schizosaccharomyces pombe | - |
- |
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
membrane | - |
Schizosaccharomyces pombe | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for maximal activity | Saccharomyces cerevisiae | |
divalent cations | required | Saccharomyces cerevisiae | |
Fe2+ | less than 10% activation | Saccharomyces cerevisiae | |
Mg2+ | activation | Saccharomyces cerevisiae | |
Mn2+ | activation, enzyme with Mn2+ is 60-95% as active as compared with Ca2+ | Saccharomyces cerevisiae | |
Mn2+ | in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ | Saccharomyces cerevisiae | |
additional information | - |
Saccharomyces cerevisiae | |
Ni2+ | less than 10% activation | Saccharomyces cerevisiae | |
Zn2+ | less than 10% activation | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
deglycosylated GDPase, result of proteolytic cleavage of the 55000 Da enzyme during purification, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | Saccharomyces cerevisiae | GDP is a reaction product following outer-chain mannosylation of luminal proteins and inhibitor of mannosyltransferases. It is hypothesized that GMP then returns to the cytosol. GDPase presumably serves as an essential function in processing of yeast glycoproteins | 5'-GMP + phosphate | - |
? | |
GDP + H2O | Saccharomyces cerevisiae | GDP is known to be an inhibitor of mannosylation reactions in the Golgi lumen, removal of GDP by conversion to GMP, the latter not being an inhibitor of these reactions, would allow mannosylation to proceed | 5'-GMP + phosphate | - |
? | |
additional information | Saccharomyces cerevisiae | role in mannosylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
partial | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3080 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP + H2O | best substrate | Saccharomyces cerevisiae | 5'-GMP + phosphate | - |
? | |
GDP + H2O | best substrate | Schizosaccharomyces pombe | 5'-GMP + phosphate | - |
? | |
GDP + H2O | highly specific for GDP | Saccharomyces cerevisiae | 5'-GMP + phosphate | - |
? | |
GDP + H2O | GDP is a reaction product following outer-chain mannosylation of luminal proteins and inhibitor of mannosyltransferases. It is hypothesized that GMP then returns to the cytosol. GDPase presumably serves as an essential function in processing of yeast glycoproteins | Saccharomyces cerevisiae | 5'-GMP + phosphate | - |
? | |
GDP + H2O | GDP is known to be an inhibitor of mannosylation reactions in the Golgi lumen, removal of GDP by conversion to GMP, the latter not being an inhibitor of these reactions, would allow mannosylation to proceed | Saccharomyces cerevisiae | 5'-GMP + phosphate | - |
? | |
additional information | substrate specificity | Saccharomyces cerevisiae | ? | - |
? | |
additional information | substrate specificity | Schizosaccharomyces pombe | ? | - |
? | |
additional information | less than 2% of maximum activity with other nucleoside di-, mono-, and triphosphates | Saccharomyces cerevisiae | ? | - |
? | |
additional information | less than 2% of maximum activity with other nucleoside di-, mono-, and triphosphates | Schizosaccharomyces pombe | ? | - |
? | |
additional information | role in mannosylation | Saccharomyces cerevisiae | ? | - |
? | |
UDP + H2O | about 10% as active with UDP compared to GDP | Saccharomyces cerevisiae | UMP + phosphate | - |
? | |
UDP + H2O | about 10% as active with UDP compared to GDP | Schizosaccharomyces pombe | UMP + phosphate | - |
? | |
UDP + H2O | in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ | Saccharomyces cerevisiae | UMP + phosphate | - |
? | |
UDP + H2O | in the presence of 10 mM Mn2+: 3fold activation of its activity toward UDP, but not GDP, compared to activation by 10 mM Ca2+ | Schizosaccharomyces pombe | UMP + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3830 | - |
GDP | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.2 | broad pH optimum | Saccharomyces cerevisiae |