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Literature summary for 3.6.1.41 extracted from

  • Hu, W.; Wang, Q.; Bi, R.
    Preparation, crystallization and preliminary X-ray crystallographic studies of diadenosine tetraphosphate hydrolase from Shigella flexneri 2a (2005), Acta Crystallogr. Sect. F, 61, 1078-1080.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(D3) Shigella flexneri

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using PEG 550 MME as precipitant Shigella flexneri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
SDS-PAGE Shigella flexneri

Organism

Organism UniProt Comment Textmining
Shigella flexneri
-
-
-
Shigella flexneri 2a
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-chelating Sepharose column chromatography, Resource-Q anion exchange chromatography and HiLoad 16/60 Superdex G75 column chromatography Shigella flexneri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diadenosine tetraphosphate + H2O
-
Shigella flexneri 2 adenosine diphosphate
-
?
diadenosine tetraphosphate + H2O
-
Shigella flexneri 2a 2 adenosine diphosphate
-
?

Subunits

Subunits Comment Organism
dimer DynaPro dynamic light scattering Shigella flexneri

Synonyms

Synonyms Comment Organism
diadenosine tetraphosphate hydrolase
-
Shigella flexneri