BRENDA - Enzyme Database
show all sequences of 3.6.1.41

Regulation of dinucleoside polyphosphate pools by the YgdP and ApaH hydrolases Is essential for the ability of Salmonella enterica serovar Typhimurium to invade cultured mammalian cells

Ismail, T.M.; Hart, C.A.; McLennan, A.G.; J. Biol. Chem. 278, 32602-32607 (2003)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli of a His-tagged recombinant protein
Salmonella enterica
Engineering
Protein Variants
Commentary
Organism
additional information
generation of null mutants by replacement of the gene encoding the enzyme with antibiotic resistance cassettes, the resulting STYA201 strain shows reduced ability to invade cultured mammalian cells
Salmonella enterica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
P1,P6-bis(5'-adenosyl)hexaphosphate
reaction medium contains 0.1 mM CoCl2
Salmonella enterica
0.037
-
P1,P4-bis(5'-adenosyl) tetraphosphate
reaction medium contains 0.1 mM CoCl2
Salmonella enterica
Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica
-
serovar typhimurium
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
hydrolysis of Ap4A
656153
Salmonella enterica
ADP
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
hydrolysis of Ap6A
656153
Salmonella enterica
ADP + ?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
ApaH hydrolase
-
Salmonella enterica
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli of a His-tagged recombinant protein
Salmonella enterica
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
generation of null mutants by replacement of the gene encoding the enzyme with antibiotic resistance cassettes, the resulting STYA201 strain shows reduced ability to invade cultured mammalian cells
Salmonella enterica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
P1,P6-bis(5'-adenosyl)hexaphosphate
reaction medium contains 0.1 mM CoCl2
Salmonella enterica
0.037
-
P1,P4-bis(5'-adenosyl) tetraphosphate
reaction medium contains 0.1 mM CoCl2
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
hydrolysis of Ap4A
656153
Salmonella enterica
ADP
-
-
-
?
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
hydrolysis of Ap6A
656153
Salmonella enterica
ADP + ?
-
-
-
?
Other publictions for EC 3.6.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733872
Sasaki
Enzymatic characteristics of a ...
Myxococcus xanthus, Myxococcus xanthus DK 1622
FEBS Lett.
588
3395-3402
2014
-
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-
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6
4
-
2
-
-
-
5
-
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11
-
4
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-
4
-
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10
-
4
-
3
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11
-
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-
4
-
-
-
-
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-
4
4
697896
Guranowski
Novel diadenosine polyphosphat ...
Escherichia coli
FEBS J.
276
1546-1553
2009
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-
-
-
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-
6
-
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1
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3
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1
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1
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1
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667066
Hu
Preparation, crystallization a ...
Shigella flexneri, Shigella flexneri 2a
Acta Crystallogr. Sect. F
61
1078-1080
2005
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-
1
1
-
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1
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6
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1
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2
1
1
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1
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1
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1
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2
1
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654033
Guranowski
Selective splitting of 3'-aden ...
Escherichia coli
Acta Biochim. Pol.
50
123-130
2003
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-
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-
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4
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2
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1
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2
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654523
Guranowski
Adenosine-5'-O-phosphorylated ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Biochem. J.
373
635-640
2003
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-
1
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22
2
-
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45
-
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1
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3
-
2
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22
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1
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22
22
2
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1
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-
3
-
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-
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-
-
656153
Ismail
Regulation of dinucleoside pol ...
Salmonella enterica
J. Biol. Chem.
278
32602-32607
2003
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-
1
-
1
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-
2
-
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1
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2
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1
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1
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1
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2
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209910
Guranowski
Regiospecificity of the hydrol ...
Escherichia coli
Biochemistry
33
235-240
1994
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2
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4
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4
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246769
McLennan
Recognition of beta beta-subst ...
Escherichia coli
Biochemistry
28
3868-3875
1989
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1
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2
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246768
Taylor
-
Phosphonate analogs of bis(5'- ...
Escherichia coli
Biochem. Soc. Trans.
16
713-714
1988
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1
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1
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1
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246767
Guranowski
Phosphonate analogues of diade ...
Escherichia coli
Biochemistry
26
3425-3429
1987
-
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-
-
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4
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3
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1
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4
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1
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246770
Plateau
Catabolism of bis(5-nucleosidy ...
Escherichia coli, Escherichia coli EM20031
Biochemistry
24
914-922
1985
-
-
-
-
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-
1
2
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3
2
-
-
3
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-
1
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1
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18
1
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1
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1
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2
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3
2
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1
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1
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18
1
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1
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246765
Guranowski
Catabolism of diadenosine 5,5- ...
Acidaminococcus fermentans, Escherichia coli, Pyrodictium occultum
J. Biol. Chem.
258
14784-14789
1983
-
-
-
-
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8
1
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6
1
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3
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1
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1
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10
-
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1
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8
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1
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6
1
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1
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1
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10
-
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1
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246763
Barnes
Isolation and characterization ...
Physarum polycephalum
Biochemistry
21
6123-6128
1982
-
-
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13
2
-
-
1
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1
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1
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1
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13
-
2
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1
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1
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1
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-
246764
Garrison
Diadenosine 5,5-P1,P4-tetrapho ...
Physarum polycephalum
Biochemistry
21
6129-6133
1982
-
-
-
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-
-
-
-
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1
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9
-
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9
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