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Literature summary for 3.6.1.41 extracted from

  • Guranowski, A.; Starzynska, E.; McLennan, A.G.; Baraniak, J.; Stec, W.J.
    Adenosine-5'-O-phosphorylated and adenosine-5'-O-phosphorothioylated polyols as strong inhibitors of (symmetrical) and (asymmetrical) dinucleoside tetraphosphatases (2003), Biochem. J., 373, 635-640.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.41

Cloned(Commentary)

Cloned (Comment) Organism
expression of a His-tagged protein in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
1,2,3,4-tetra(adenosine-5'-O-phospho) erythritol
-
 Escherichia coli
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol competitive with respect to Ap4A Escherichia coli
1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1,2,3-tri(adenosine-5'-O-phospho) glycerol
-
 Escherichia coli
1,3-di(adenosine-5'-O-phospho) glycerol
-
 Escherichia coli
1,3-di(adenosine-5'-O-phospho)-2-phosphoglycerol
-
 Escherichia coli
1,3-di(adenosine-5'-O-phosphorothio) glycerol
-
 Escherichia coli
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol
-
 Escherichia coli
1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1,4-di(adenosine-5'-O-phosphorothio) butanediol
-
 Escherichia coli
1,4-di(adenosine-5'-O-phosphorothio) erythritol
-
 Escherichia coli
1,5-di(adenosine-5'-O-phosphorothio) pentanediol
-
 Escherichia coli
di(adenosine-5'-O-phospho)-di(phospho) pentaerythritol
-
 Escherichia coli
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol competitive with respect to Ap4A Escherichia coli
di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol
-
 Salmonella enterica subsp. enterica serovar Typhimurium
tetra(adenosine-5'-O-phospho) pentaerythritol
-
 Escherichia coli
tetra(adenosine-5'-O-phosphorothio) pentaerythritol
-
 Escherichia coli
tetra(adenosine-5'-O-phosphorothio) pentaerythritol
-
 Salmonella enterica subsp. enterica serovar Typhimurium
tri(adenosine-5'-O-phospho)-phospho pentaerythritol
-
 Escherichia coli
tri(adenosine-5'-O-phosphorothio) glycerol
-
 Escherichia coli
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol
-
 Escherichia coli
tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 P1,P4-bis(5'-adenosyl) tetraphosphate pH 7.6, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
0.025
-
 P1,P4-bis(5'-adenosyl) tetraphosphate pH 7.6, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K12 strain
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT2 strain
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-affinity column chromatography to isolate a His-tagged recombinant enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2 ADP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O hydrolysis of Ap4A Escherichia coli 2 ADP
-
 ?

Synonyms

Synonyms Comment Organism
(symmetrical) Ap4A hydrolase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
(symmetrical) Ap4A hydrolase
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
 di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol pH 7.6, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
0.00004
-
 di(adenosine-5'-O-phosphorothio)-di(phosphorothio) pentaerythritol pH 7.6, 30°C Escherichia coli
0.00008
-
 tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol pH 7.6, 30°C Escherichia coli
0.001
-
 di(adenosine-5'-O-phospho)-di(phospho) pentaerythritol pH 7.6, 30°C Escherichia coli
0.0013
-
 1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol pH 7.6, 30°C Escherichia coli
0.0014
-
 1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol pH 7.6, 30°C Escherichia coli
0.0014
-
 tetra(adenosine-5'-O-phosphorothio) pentaerythritol pH 7.6, 30°C Escherichia coli
0.0017
-
 tri(adenosine-5'-O-phosphorothio)-phosphorothio pentaerythritol pH 7.6, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
0.0018
-
 tetra(adenosine-5'-O-phosphorothio) pentaerythritol pH 7.6, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
0.0029
-
 1,2,3,4-tetra(adenosine-5'-O-phosphorothio) erythritol pH 7.6, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
0.003
-
 tri(adenosine-5'-O-phospho)-phospho pentaerythritol pH 7.6, 30°C Escherichia coli
0.0039
-
 1,3-di(adenosine-5'-O-phosphorothio)-2-phosphorothioglycerol pH 7.6, 30°C Salmonella enterica subsp. enterica serovar Typhimurium
0.005
-
 1,3-di(adenosine-5'-O-phospho)-2-phosphoglycerol pH 7.6, 30°C Escherichia coli
0.006
-
 1,2,3,4-tetra(adenosine-5'-O-phospho) erythritol pH 7.6, 30°C Escherichia coli
0.007
-
 tri(adenosine-5'-O-phosphorothio) glycerol pH 7.6, 30°C Escherichia coli
0.028
-
 tetra(adenosine-5'-O-phospho) pentaerythritol pH 7.6, 30°C Escherichia coli
0.078
-
 1,4-di(adenosine-5'-O-phosphorothio) erythritol pH 7.6, 30°C Escherichia coli
0.089
-
 1,4-di(adenosine-5'-O-phosphorothio) butanediol pH 7.6, 30°C Escherichia coli
0.109
-
 1,5-di(adenosine-5'-O-phosphorothio) pentanediol pH 7.6, 30°C Escherichia coli
0.157
-
 1,3-di(adenosine-5'-O-phosphorothio) glycerol pH 7.6, 30°C Escherichia coli
0.215
-
 1,3-di(adenosine-5'-O-phospho) glycerol pH 7.6, 30°C Escherichia coli
4.08
-
 1,2,3-tri(adenosine-5'-O-phospho) glycerol pH 7.6, 30°C Escherichia coli