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Literature summary for 3.6.1.41 extracted from

  • Plateau, P.; Fromant, M.; Brevet, A.; Gesquiere, A.; Blanquet, S.
    Catabolism of bis(5-nucleosidyl) oligophosphates in Escherichia coli: metal requirements and substrate specificity of homogeneous diadenosine-5,5-P1,P4-tetraphosphate pyrophosphohydrolase (1985), Biochemistry, 24, 914-922.
    View publication on PubMed
Search for more literature for 3.6.1.41

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ exposure prior to the assay Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 P1,P4-bis(5'-adenosyl) tetraphosphate with Mn2+ Escherichia coli
0.022
-
 P1,P4-bis(5'-adenosyl) tetraphosphate with Co2+ Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Escherichia coli
Co2+ 2500fold stimulation Escherichia coli
Mn2+ 900fold stimulation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 gel filtration Escherichia coli
34000
-
 1 * 34000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 EM20031
-
Escherichia coli EM20031
-
 EM20031
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 5'-P(1)-tetraphosphate-P(4)-5'''-cytidine + H2O
-
 Escherichia coli ADP + CDP
-
 ?
adenosine 5'-P(1)-triphosphate-P(3)-5''-cytidine + H2O
-
 Escherichia coli ADP + CMP + CDP + AMP
-
 ?
adenosine 5'-P(1)-triphosphate-P(3)-5''-guanosine + H2O
-
 Escherichia coli ADP + GMP
-
 ?
Ap3A + H2O
-
 Escherichia coli ADP + AMP
-
 ?
Ap3A + H2O
-
 Escherichia coli EM20031 ADP + AMP
-
 ?
Ap4 + H2O
-
 Escherichia coli ?
-
 ?
Ap4G + H2O
-
 Escherichia coli ADP + GDP
-
 ?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
 Escherichia coli ADP + ATP
-
 ?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
 Escherichia coli EM20031 ADP + ATP
-
 ?
Gp3C + H2O
-
 Escherichia coli CDP + GMP
-
 ?
Gp3G + H2O
-
 Escherichia coli guanosine 3'-diphosphate + guanosine 5'-phosphate
-
 ?
P1,P4-bis(5'-(2'-deoxy)adenosyl)tetraphosphate + H2O
-
 Escherichia coli 2 dADP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Escherichia coli ADP
-
 ?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Escherichia coli EM20031 ADP
-
 ?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
 Escherichia coli GTP
-
 ?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
 Escherichia coli EM20031 GTP
-
 ?
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
 Escherichia coli ADP + adenosine 5'-tetraphosphate
-
 ?
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
 Escherichia coli EM20031 ADP + adenosine 5'-tetraphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
250
-
 P1,P4-bis(5'-adenosyl) tetraphosphate
-
 Escherichia coli