BRENDA - Enzyme Database
show all sequences of 3.6.1.41

Catabolism of diadenosine 5,5-P1,P4-tetraphosphate in procaryotes. Purification and properties of diadenosine 5,5-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12

Guranowski, A.; Jakubowski, H.; Holler, E.; J. Biol. Chem. 258, 14784-14789 (1983)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Escherichia coli
Cu2+
-
Acidaminococcus fermentans
Cu2+
no effect below 0.03 mM, inhibition above
Escherichia coli
Cu2+
-
Pyrodictium occultum
Fe2+
no effect below 0.03 mM, inhibition above
Acidaminococcus fermentans
Fe2+
no effect below 0.03 mM, inhibition above
Escherichia coli
Fe2+
no effect below 0.03 mM, inhibition above
Pyrodictium occultum
Zn2+
no effect below 0.03 mM, inhibition above
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentration
Escherichia coli
Cd2+
0.002-0.005 mM, 3.5fold stimulation
Escherichia coli
Co2+
0.1-0.5 mM, 100fold increase of activity
Escherichia coli
Mg2+
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations
Escherichia coli
Mn2+
0.2-0.5 mM, 8fold stimulation
Escherichia coli
Ni2+
0.004-0.008 mM, 3.5fold stimulation
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
gel filtration
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acidaminococcus fermentans
-
-
-
Escherichia coli
-
K12
-
Pyrodictium occultum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.062
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Ap3A + H2O
-
246765
Escherichia coli
ADP + AMP
-
-
-
?
Ap4 + H2O
-
246765
Escherichia coli
?
-
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
246765
Escherichia coli
ADP + ATP
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Escherichia coli
ADP
-
246765
Escherichia coli
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Acidaminococcus fermentans
ADP
-
246765
Acidaminococcus fermentans
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Pyrodictium occultum
ADP
-
246765
Pyrodictium occultum
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Escherichia coli
GTP
-
-
-
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Acidaminococcus fermentans
GTP
-
-
-
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Pyrodictium occultum
GTP
-
-
-
?
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
246765
Escherichia coli
ADP + adenosine 5'-tetraphosphate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Escherichia coli
Cu2+
-
Acidaminococcus fermentans
Cu2+
no effect below 0.03 mM, inhibition above
Escherichia coli
Cu2+
-
Pyrodictium occultum
Fe2+
no effect below 0.03 mM, inhibition above
Acidaminococcus fermentans
Fe2+
no effect below 0.03 mM, inhibition above
Escherichia coli
Fe2+
no effect below 0.03 mM, inhibition above
Pyrodictium occultum
Zn2+
no effect below 0.03 mM, inhibition above
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentration
Escherichia coli
Cd2+
0.002-0.005 mM, 3.5fold stimulation
Escherichia coli
Co2+
0.1-0.5 mM, 100fold increase of activity
Escherichia coli
Mg2+
weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations
Escherichia coli
Mn2+
0.2-0.5 mM, 8fold stimulation
Escherichia coli
Ni2+
0.004-0.008 mM, 3.5fold stimulation
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.062
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Ap3A + H2O
-
246765
Escherichia coli
ADP + AMP
-
-
-
?
Ap4 + H2O
-
246765
Escherichia coli
?
-
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
246765
Escherichia coli
ADP + ATP
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Escherichia coli
ADP
-
246765
Escherichia coli
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Acidaminococcus fermentans
ADP
-
246765
Acidaminococcus fermentans
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
246765
Pyrodictium occultum
ADP
-
246765
Pyrodictium occultum
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Escherichia coli
GTP
-
-
-
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Acidaminococcus fermentans
GTP
-
-
-
?
P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O
-
246765
Pyrodictium occultum
GTP
-
-
-
?
P1,P6-di(adenosine-5')hexaphosphate + H2O
-
246765
Escherichia coli
ADP + adenosine 5'-tetraphosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Escherichia coli
Other publictions for EC 3.6.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733872
Sasaki
Enzymatic characteristics of a ...
Myxococcus xanthus, Myxococcus xanthus DK 1622
FEBS Lett.
588
3395-3402
2014
-
-
-
-
-
-
6
4
-
2
-
-
-
5
-
-
-
-
-
-
-
-
11
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
4
-
3
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
697896
Guranowski
Novel diadenosine polyphosphat ...
Escherichia coli
FEBS J.
276
1546-1553
2009
-
-
-
-
-
-
6
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667066
Hu
Preparation, crystallization a ...
Shigella flexneri, Shigella flexneri 2a
Acta Crystallogr. Sect. F
61
1078-1080
2005
-
-
1
1
-
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654033
Guranowski
Selective splitting of 3'-aden ...
Escherichia coli
Acta Biochim. Pol.
50
123-130
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654523
Guranowski
Adenosine-5'-O-phosphorylated ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Biochem. J.
373
635-640
2003
-
-
1
-
-
-
22
2
-
-
-
-
-
43
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
22
-
-
-
-
1
-
-
-
-
-
22
22
2
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656153
Ismail
Regulation of dinucleoside pol ...
Salmonella enterica
J. Biol. Chem.
278
32602-32607
2003
-
-
1
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209910
Guranowski
Regiospecificity of the hydrol ...
Escherichia coli
Biochemistry
33
235-240
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246769
McLennan
Recognition of beta beta-subst ...
Escherichia coli
Biochemistry
28
3868-3875
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246768
Taylor
-
Phosphonate analogs of bis(5'- ...
Escherichia coli
Biochem. Soc. Trans.
16
713-714
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
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-
1
-
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-
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-
-
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-
-
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246767
Guranowski
Phosphonate analogues of diade ...
Escherichia coli
Biochemistry
26
3425-3429
1987
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246770
Plateau
Catabolism of bis(5-nucleosidy ...
Escherichia coli, Escherichia coli EM20031
Biochemistry
24
914-922
1985
-
-
-
-
-
-
1
2
-
3
2
-
-
3
-
-
1
-
-
-
1
-
18
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
3
2
-
-
-
-
1
-
-
1
-
18
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
246765
Guranowski
Catabolism of diadenosine 5,5- ...
Acidaminococcus fermentans, Escherichia coli, Pyrodictium occultum
J. Biol. Chem.
258
14784-14789
1983
-
-
-
-
-
-
8
1
-
6
1
-
-
3
-
-
1
-
-
-
1
-
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
1
-
6
1
-
-
-
-
1
-
-
1
-
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
246763
Barnes
Isolation and characterization ...
Physarum polycephalum
Biochemistry
21
6123-6128
1982
-
-
-
-
-
-
13
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
2
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246764
Garrison
Diadenosine 5,5-P1,P4-tetrapho ...
Physarum polycephalum
Biochemistry
21
6129-6133
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-