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Literature summary for 3.6.1.41 extracted from
- Catabolism of diadenosine 5,5-P1,P4-tetraphosphate in procaryotes. Purification and properties of diadenosine 5,5-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12 (1983), J. Biol. Chem., 258, 14784-14789.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Escherichia coli |  |
| Cu2+ | - |
Acidaminococcus fermentans | |
| Cu2+ | no effect below 0.03 mM, inhibition above | Escherichia coli | |
| Cu2+ | - |
Pyrodictium occultum | |
| Fe2+ | no effect below 0.03 mM, inhibition above | Acidaminococcus fermentans | |
| Fe2+ | no effect below 0.03 mM, inhibition above | Escherichia coli | |
| Fe2+ | no effect below 0.03 mM, inhibition above | Pyrodictium occultum | |
| Zn2+ | no effect below 0.03 mM, inhibition above | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentration | Escherichia coli | |
| Cd2+ | 0.002-0.005 mM, 3.5fold stimulation | Escherichia coli | |
| Co2+ | 0.1-0.5 mM, 100fold increase of activity | Escherichia coli | |
| Mg2+ | weak stimulation in absence of other divalent metal ions, high synergistic stimulation in combination with Co2+ at its suboptimal concentrations | Escherichia coli | |
| Mn2+ | 0.2-0.5 mM, 8fold stimulation | Escherichia coli | |
| Ni2+ | 0.004-0.008 mM, 3.5fold stimulation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidaminococcus fermentans | - |
- |
- |
| Escherichia coli | - |
K12 | - |
| Pyrodictium occultum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.062 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ap3A + H2O | - |
Escherichia coli | ADP + AMP | - |
? | |
| Ap4 + H2O | - |
Escherichia coli | ? | - |
? | |
| diadenosine 5',5''-P1,P5-pentaphosphate + H2O | - |
Escherichia coli | ADP + ATP | - |
? | |
| P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Escherichia coli | ADP | - |
? | |
| P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Acidaminococcus fermentans | ADP | - |
? | |
| P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Pyrodictium occultum | ADP | - |
? | |
| P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O | - |
Escherichia coli | GTP | - |
? | |
| P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O | - |
Acidaminococcus fermentans | GTP | - |
? | |
| P1,P4-Bis(5'-guanosyl) tetraphosphate + H2O | - |
Pyrodictium occultum | GTP | - |
? | |
| P1,P6-di(adenosine-5')hexaphosphate + H2O | - |
Escherichia coli | ADP + adenosine 5'-tetraphosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Escherichia coli |
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