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Literature summary for 3.6.1.40 extracted from

  • Kristensen, O.; Ross, B.; Gajhede, M.
    Structure of the PPX/GPPA phosphatase from Aquifex aeolicus in complex with the alarmone ppGpp (2008), J. Mol. Biol., 375, 1469-1476.
    View publication on PubMed
Search for more literature for 3.6.1.40

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged Y19N mutant Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment) Organism
PPX/GPPA Y19N mutant enzyme in complex with guanosine tetraphosphate, hanging-drop vapor diffusion method by mixing 0.002 ml of protein and 0.002 ml of reservoir solution containing 200 mM sodium acetate, pH 6.4, 100 mM 4-morpholineethanesulfonic acid, pH 5.7, and 50% methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 2.7 A resolution, modelling Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
Y19N site-directed mutagenesis, crystal structure Aquifex aeolicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanosine 5'-triphosphate,3'-diphosphate + H2O Aquifex aeolicus
-
 guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Y19N mutant by nickel affinity chromatography removal of His-tag, and gel filtration Aquifex aeolicus

Reaction

Reaction Comment Organism Reaction ID
guanosine 5'-triphosphate 3'-diphosphate + H2O = guanosine 3',5'-bis(diphosphate) + phosphate the hydrolytic base is E119, the dual specificity typical for the exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family is due to a common active site for guanosine pentaphosphate and polyphosphate hydrolysis, R22 and R267, residing in different domains, are crucial for guanosine pentaphosphate specificity as they interact with the unique 3'-ribose phosphorylation Aquifex aeolicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
 Aquifex aeolicus guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
 ?
guanosine 5'-triphosphate,3'-diphosphate + H2O substrate binding structure and involved residues, overview Aquifex aeolicus guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
exopolyphosphatase/guanosine pentaphosphate phosphohydrolase
-
 Aquifex aeolicus
More the enzyme belongs to the exopolyphosphatase/guanosine pentaphosphate phosphohydrolase protein family Aquifex aeolicus
PPX/GPPA enzyme
-
 Aquifex aeolicus