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Literature summary for 3.6.1.39 extracted from
- Coupling of DNA unwinding to nucleotide hydrolysis in a ring-shaped helicase (2008), EMBO J., 27, 1718-1726.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | dTTP hydrolysis kinetics are measured in real time using the phosphate-binding protein that acts as a sensitive sensor for fast detection of low concentrations of phosphate, overview | Escherichia phage T7 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + H2O | Escherichia phage T7 | dTTP hydrolysis is coupled to DNA unwinding, dependence of dTTP hydrolysis rates on dsDNA stability, the force-producing step during DNA unwinding is not associated with dTTP binding, but dTTP hydrolysis or phosphate release | dTDP + phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia phage T7 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + H2O | dTTP hydrolysis is coupled to DNA unwinding, dependence of dTTP hydrolysis rates on dsDNA stability, the force-producing step during DNA unwinding is not associated with dTTP binding, but dTTP hydrolysis or phosphate release | Escherichia phage T7 | dTDP + phosphate | - |
? | |
| dTTP + H2O | dTTP as part of DNA molecules, activity assay using four different DNA substrates with the same GC content of 5%, but varying lengths of duplex regions, fluorescent substrates, mechanochemistry and kinetics of the DNA-unwinding reaction, overview | Escherichia phage T7 | dTDP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dTTPase | - |
Escherichia phage T7 |
| T7 helicase | - |
Escherichia phage T7 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | - |
assay at | Escherichia phage T7 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Escherichia phage T7 |
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Release 2023.1 (January 2023)
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