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Literature summary for 3.6.1.39 extracted from

  • Dahlmann, N.
    Human serum thymidine triphosphate nucleotidohydrolase: purification and properties of a new enzyme (1982), Biochemistry, 21, 6634-6639.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA with 0.2-2.0 mM EDTA 80% loss of activity Homo sapiens
Zn2+ causes complete inhibition in absence of EDTA Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
dTTP
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Mg2+
-
Homo sapiens
Mn2+ reverses inhibitory effect of EDTA Homo sapiens
Zn2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
SDS-PAGE Homo sapiens
48500
-
gel filtration and sucrose density gradient centrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTTP + H2O Homo sapiens
-
dTDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogenity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTTP + H2O
-
Homo sapiens dTDP + phosphate
-
?
dUTP + H2O 50% activity compared with dTTP as substrate Homo sapiens dUDP + phosphate
-
?
UTP + H2O 20% activity compared with dTTP as substrate Homo sapiens UDP + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-Page, gel filtration Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
12 23 no loss of activity within 1 h Homo sapiens
30 37 stability starts to decline above 30°C and breaks down at 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Homo sapiens