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Literature summary for 3.6.1.29 extracted from

  • Huang, K.; Arabshahi, A.; Wei, Y.; Frey, P.A.
    The mechanism of action of the fragile histidine triad, Fhit: isolation of a covalent adenylyl enzyme and chemical rescue of H96G-Fhit (2004), Biochemistry, 43, 7637-7642.
    View publication on PubMed
Search for more literature for 3.6.1.29

Protein Variants

Protein Variants Comment Organism
H96G site-directed mutagenesis in the fragile histidine triad, leads to reduced activity and reduced hydrolysis of reaction intermediates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0029
-
 P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate wild-type enzyme, pH 7.3 Homo sapiens
0.0053
-
 adenosine-5'-phospho-N-methylimidazolide wild-type enzyme, pH 6.6 Homo sapiens
0.0062
-
 adenosine-5'-phosphoimidazolide wild-type enzyme, pH 5.8 Homo sapiens
0.026
-
 adenosine-5'-phosphoimidazolide mutant H96G, pH 5.8 Homo sapiens
0.026
-
 P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate mutant H96G, pH 8.9 Homo sapiens
0.18
-
 adenosine-5'-phospho-N-methylimidazolide mutant H96G, pH 6.6 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, formation of an MgADP- intermediate during catalytic reaction Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O Homo sapiens
-
 AMP + ADP
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP two-step reaction mechanism via a fragile histidine triad Fhit-AMP intermediate and release of MgADP-, kinetic of the reaction steps, overview Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine-5'-phospho-N-methylimidazolide + H2O
-
 Homo sapiens ?
-
 ?
adenosine-5'-phosphoimidazolide + H2O
-
 Homo sapiens ?
-
 ?
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O
-
 Homo sapiens AMP + ADP
-
 r

Synonyms

Synonyms Comment Organism
covalent adenylyl enzyme
-
 Homo sapiens
Fhit
-
 Homo sapiens
fragile histidine triad
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0014
-
 P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate mutant H96G, pH 8.9 Homo sapiens
0.62
-
 adenosine-5'-phospho-N-methylimidazolide mutant H96G, pH 6.6 Homo sapiens
0.91
-
 adenosine-5'-phosphoimidazolide mutant H96G, pH 5.8 Homo sapiens
3.5
-
 P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate wild-type enzyme, pH 7.3 Homo sapiens
4.2
-
 adenosine-5'-phospho-N-methylimidazolide wild-type enzyme, pH 6.6 Homo sapiens
4.4
-
 adenosine-5'-phosphoimidazolide wild-type enzyme, pH 5.8 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 optimal pH for hydrolysis of adenosine-5'-phosphoimidazolide Homo sapiens
6.6
-
 optimal pH for hydrolysis of adenosine-5'-phospho-N-methylimidazolide Homo sapiens
7.3
-
 optimal pH for hydrolysis of P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate Homo sapiens