BRENDA - Enzyme Database
show all sequences of 3.6.1.29

Fith-nucleotide specificity probed with novel fluorescent and fluorogenic substrates

Draganescu, A.; Hodawadekar, S.C.; Gee, K.R.; Brenner, C.; J. Biol. Chem. 275, 4555-4560 (2000)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Homo sapiens
0.0026
-
Ap4A
-
Homo sapiens
0.013
-
Ap5A
-
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
P1-P3-bis(5'-adenosyl) triphosphate + H2O
Homo sapiens
-
ADP + AMP
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Appp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
-
289066
Homo sapiens
AMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
-
-
-
?
Appp-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin + H2O
-
289066
Homo sapiens
AMP + diphospho-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin
-
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
289066
Homo sapiens
AMP + ATP
-
-
-
-
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
289066
Homo sapiens
AMP + adenosine 5'-tetraphosphate
-
-
-
-
Gppp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
-
289066
Homo sapiens
GMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
289066
Homo sapiens
ADP + AMP
-
-
-
?
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0019
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Homo sapiens
0.0026
-
Ap4A
-
Homo sapiens
0.013
-
Ap5A
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
P1-P3-bis(5'-adenosyl) triphosphate + H2O
Homo sapiens
-
ADP + AMP
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Appp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
-
289066
Homo sapiens
AMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
-
-
-
?
Appp-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin + H2O
-
289066
Homo sapiens
AMP + diphospho-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin
-
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
289066
Homo sapiens
AMP + ATP
-
-
-
-
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
289066
Homo sapiens
AMP + adenosine 5'-tetraphosphate
-
-
-
-
Gppp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
-
289066
Homo sapiens
GMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
289066
Homo sapiens
ADP + AMP
-
-
-
?
Other publictions for EC 3.6.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718972
Martin
Hit proteins, mitochondria and ...
Homo sapiens
Biochim. Biophys. Acta
1807
626-632
2011
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
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1
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-
2
-
-
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-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
719088
Krakowiak
Evaluation of influence of Ap4 ...
Homo sapiens
Bioorg. Med. Chem.
19
5053-5060
2011
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720558
Guranowski
-
Recognition of different nucle ...
Arabidopsis thaliana, Homo sapiens, Trypanosoma brucei
New J. Chem.
34
888-893
2010
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
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-
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21
-
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3
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21
-
-
-
-
-
-
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-
-
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-
-
-
-
-
699134
Mirandola
TRAIL-induced apoptosis of FHI ...
Homo sapiens
J. Cell. Physiol.
220
493-498
2009
-
-
1
-
1
-
1
-
-
-
-
2
-
2
-
-
-
-
-
4
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
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-
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-
2
-
-
-
-
-
4
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713502
Banerjee
Identification of the HIT-45 p ...
Trypanosoma brucei, Trypanosoma brucei 427
RNA
15
1554-1564
2009
-
-
1
-
1
-
-
-
-
-
-
-
-
12
-
-
1
-
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697934
Guranowski
Fhit proteins can also recogni ...
Arabidopsis thaliana, Homo sapiens
FEBS Lett.
582
3152-3158
2008
-
-
1
-
-
-
11
2
-
2
-
11
-
2
-
-
-
-
-
-
2
-
20
-
2
-
-
2
2
-
-
-
10
-
-
-
-
1
-
-
-
-
-
11
10
2
-
2
-
11
-
-
-
-
-
-
2
-
20
-
2
-
-
2
2
-
-
-
-
-
-
-
-
-
667847
Asensio
Biochemical and immunochemical ...
Homo sapiens, Rattus norvegicus
Biochimie
88
461-471
2006
-
-
-
-
-
-
3
1
1
3
1
2
-
4
-
-
2
-
-
4
1
-
2
1
1
-
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
2
3
1
1
1
3
1
2
-
-
-
2
-
4
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668503
Nakagawa
Fhit protein inhibits cell gro ...
Homo sapiens
Exp. Cell Res.
312
2433-2442
2006
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668869
Zhao
Fhit protein is preferentially ...
Homo sapiens
Histol. Histopathol.
21
915-923
2006
-
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
12
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
12
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667199
Di Meo
Comparative mapping of the fra ...
Bos taurus
Anim. Genet.
36
363-365
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
667667
Garrison
Phosphorylation of the human F ...
Homo sapiens
Biochemistry
44
6286-6292
2005
-
-
1
-
-
-
-
4
-
2
-
1
-
1
-
1
1
1
-
-
-
-
1
1
1
-
-
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
2
-
1
-
-
1
1
-
-
-
-
1
1
1
-
-
4
1
-
-
-
-
-
-
-
-
-
668986
Oezkara
FHIT expression in neoplastic, ...
Homo sapiens
Int. J. Gynecol. Cancer
15
1081-1088
2005
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
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-
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-
2
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655798
Huang
Engineering human Fhit, a diad ...
Homo sapiens
J. Am. Chem. Soc.
126
9548-9549
2004
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667612
Huang
The mechanism of action of the ...
Homo sapiens
Biochemistry
43
7637-7642
2004
-
-
-
-
1
-
-
6
-
1
-
1
-
2
-
-
-
1
-
-
1
-
3
-
-
-
-
6
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
1
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
6
3
-
-
-
-
-
-
-
-
-
289062
Pawelczyk
Expression in Escherichia coli ...
Homo sapiens
Protein Expr. Purif.
18
320-326
2000
-
-
1
-
-
-
-
1
-
-
1
1
-
3
-
-
1
-
-
-
1
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
1
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289066
Draganescu
Fith-nucleotide specificity pr ...
Homo sapiens
J. Biol. Chem.
275
4555-4560
2000
-
-
-
-
-
-
-
3
-
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
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-
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-
-
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-
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3
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-
1
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-
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6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289065
Pace
Genetic, biochemical and cryst ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
95
5484-5489
1998
-
-
-
-
-
-
-
1
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
1
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1
-
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-
-
-
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-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289068
Rotllan
Potent inhibition of specific ...
Rattus norvegicus
FEBS Lett.
429
143-146
1998
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
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-
-
1
-
-
1
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-
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1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289061
Barnes
Fhit, a putative tumor supress ...
Homo sapiens
Biochemistry
35
11529-11535
1996
-
-
1
-
-
-
1
2
-
3
-
1
-
2
-
-
-
-
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
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-
1
-
2
-
3
-
1
-
-
-
-
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289070
Guranowski
Dinucleoside 5',5'''-P1,P3-tri ...
Lupinus luteus
Protein Expr. Purif.
8
416-422
1996
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
11
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
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-
1
1
-
-
-
-
-
-
-
-
11
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289064
Guranowski
-
Methylene and halomethylene an ...
Lupinus luteus
Nucleosides Nucleotides
14
731-734
1995
-
-
-
-
-
-
-
4
-
-
-
1
-
1
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1
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4
-
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4
-
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-
1
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1
-
-
4
-
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-
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-
-
-
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-
-
-
-
289063
Prescott
Characterization of a bis(5'-n ...
Artemia sp.
Int. J. Biochem.
24
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Brevet
Isolation and characterization ...
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J. Bacteriol.
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1991
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289069
Garcia-Ag·ndez
Particulate diadenosine 5',5'' ...
Rattus norvegicus
Biochim. Biophys. Acta
1073
402-409
1991
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289071
Bernet
Location of dinucleoside triph ...
Rattus norvegicus
FEBS Lett.
283
286-288
1991
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289060
Hurtado
Specific magnesium-dependent d ...
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J. Bacteriol.
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1987
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209899
Costas
Mitochondrial location of rat ...
Rattus norvegicus
J. Biol. Chem.
261
2064-2067
1986
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289059
Montero
-
Hydrolytic activities on diade ...
Rattus norvegicus
Cienc. Biol.
11
1-6
1986
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208383
Luthje
Catabolism of Ap3A and Ap4A in ...
Homo sapiens
Eur. J. Biochem.
149
119-127
1985
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289057
Costas
Occurence of nucleosidetriphos ...
Rattus norvegicus
Int. J. Biochem.
17
903-909
1985
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289054
Michels
Catabolism of capped (3'-5')- ...
Rattus norvegicus
FEBS Lett.
166
57-61
1984
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289055
Costas
Dinucleosidetriphosphatase fro ...
Rattus norvegicus
Int. J. Biochem.
16
757-762
1984
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208361
Jakubowski
Enzymes hydrolyzing ApppA and/ ...
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J. Biol. Chem.
258
9982-9989
1983
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289052
Gunther Sillero
Dinucleosidetriphosphatase fro ...
Rattus norvegicus
Eur. J. Biochem.
76
331-337
1977
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