Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, the recombinant enzyme is completely functional | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
([2-[3-(4-Amino-2-methyl-pyrimidin-5-ylmethyl)-4-methyl-thiazol-5-yl]-ethoxy]-phosphonomethyl-phosphinoylmethyl)-phosphonic acid | - |
Homo sapiens | |
ATP | very poor inhibitor | Homo sapiens | |
Ca2+ | inhibits by competition with Mg2+ | Homo sapiens | |
SDS | IC50: about 0.3% W/v. The inhibition is partially reversible, probably due to correct refolding of the denatured enzyme | Homo sapiens | |
thiamine triphosphate | - |
Homo sapiens | |
Zn2+ | inhibits at micromolar concentrations at pH 8.0, IC50: about 0.015-0.02 mM. Activates at pH 6.0 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.154 | - |
thiamine triphosphate | untagged recombinant enzyme | Homo sapiens | |
3.7 | - |
ATP | in presence of 8 mM ATP | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Zn2+ | inhibits at micromolar concentrations at pH 8.0, activates at pH 6.0 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | the catalytic efficiency is about 4 orders of magnitude lower than for thiamine triphosphate | Homo sapiens | ADP + phosphate | - |
? | |
thiamine triphosphate + H2O | - |
Homo sapiens | thiamine diphosphate + phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | 30 min, stable | Homo sapiens |
68 | - |
30 min, 50% loss of activity | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
ATP | in presence of 8 mM Mg2+ | Homo sapiens | |
140 | - |
thiamine triphosphate | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | pH 7.0: about 60% of maximal activity, pH 9.5: about 75% of maximal activity | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
thiamine triphosphate | - |
Homo sapiens | |
1.2 | - |
([2-[3-(4-Amino-2-methyl-pyrimidin-5-ylmethyl)-4-methyl-thiazol-5-yl]-ethoxy]-phosphonomethyl-phosphinoylmethyl)-phosphonic acid | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.015 | 0.02 | inhibits at micromolar concentrations at pH 8.0, IC50: about 0.015-0.02 mM. Activates at pH 6.0 | Homo sapiens | Zn2+ |