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Literature summary for 3.6.1.28 extracted from
- Thiamine triphosphatases in brain (1974), Thiamine (Proc. Pap. U. S. -Jpn. Semin, 2. Meeting), , 195-212.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 1 mM, strongly inhibits membrane-associated enzyme, soluble enzyme is unaffected | Rattus norvegicus |  |
| ATP | 1 mM, strongly inhibits membrane-associated enzyme, soluble enzyme is unaffected | Rattus norvegicus | |
| Ca2+ | 0.1-1.0 mM, in presence of Mg2+, soluble enzymem | Rattus norvegicus | |
| thiamine diphosphate | soluble enzyme, at levels of substrate 2 to 3 times Km, no inhibition of membrane-associated enzyme | Rattus norvegicus | |
| thiamine triphosphate | substrate inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
Mg2+-thiamine triphosphate complex | - |
Rattus norvegicus | |
| 1.2 | - |
thiamine triphosphate | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ | Rattus norvegicus | |
| Ca2+ | activates membrane-associated enzyme | Rattus norvegicus | |
| Mg2+ | maximal activation occurs at a cation to substrate ratio of 1:1 | Rattus norvegicus | |
| Mg2+ | divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ | Rattus norvegicus | |
| Mn2+ | divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine triphosphate + H2O | - |
Rattus norvegicus | thiamine diphosphate + phosphate | - |
? | |
| thiamine triphosphate + H2O | thiamine triphosphate as Mg2+-thiamine triphosphate complex | Rattus norvegicus | thiamine diphosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
soluble enzyme | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
rapid inactivation of membrane-associated enzyme | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | membrane-associated enzyme | Rattus norvegicus |
| 9 | 9.5 | soluble enzyme | Rattus norvegicus |
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