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Literature summary for 3.6.1.28 extracted from

  • Barchi, R.L.; Braun, P.E.
    A membrane-associated thiamine triphosphatase from rat brain. Properties of the enzyme (1972), J. Biol. Chem., 247, 7668-7673.
    View publication on PubMed
Search for more literature for 3.6.1.28

Inhibitors

Inhibitors Comment Organism Structure
ADP 0.001-1 mM, strong inhibition Rattus norvegicus
deoxycholate
-
 Rattus norvegicus
thiamine beta,gamma-methylene phosphonate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 thiamine triphosphate soluble enzyme, activated by Mg2+ Rattus norvegicus
1.5
-
 thiamine triphosphate membrane-associated enzyme, activated by Ca2+ or Mg2+ Rattus norvegicus
2
-
 thiamine triphosphate soluble enzyme, activated by Ca2+ Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane associated Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Br- stimulation by anions with the following order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme Rattus norvegicus
Ca2+ maximal activation occurs at a cation to substrate ratio of 1:1 Rattus norvegicus
Ca2+ divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ Rattus norvegicus
Cl- stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme Rattus norvegicus
I- stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme Rattus norvegicus
Mg2+ maximal activation occurs at a cation to substrate ratio of 1:1 Rattus norvegicus
Mg2+ divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ Rattus norvegicus
Mn2+ maximal activation occurs at a cation to substrate ratio of 1:1 Rattus norvegicus
Mn2+ divalent cation requirement is satisfied by Mg2+, Mn2+ or Ca2+ Rattus norvegicus
NO3- stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme. The effect of NO3- is maximal at pH 7-8, it is negligible at pH 6.5 Rattus norvegicus
SCN- stimulation by anions with the following decreasing order of effectiveness: SCN-, I-, NO3-, Br-, Cl-, membrane-associated enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 enzyme from liver and brain, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
heart
-
 Rattus norvegicus
-
intestine
-
 Rattus norvegicus
-
kidney
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
skeletal muscle
-
 Rattus norvegicus
-
spleen
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiamine triphosphate + H2O
-
 Rattus norvegicus thiamine diphosphate + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 50% inactivation after 2-4 min Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 membrane-associated enzyme Rattus norvegicus
9.5
-
 soluble enzyme Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 about 55% of maximal activity at pH 5.0 and pH 8.0, membrane-associated enzyme Rattus norvegicus
8 10 pH 8.0: about 60% of maximal activity, pH 10.0: about 75% of maximal activity Rattus norvegicus