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Literature summary for 3.6.1.27 extracted from
- Substrate specificity and membrane topology of Escherichia coli PgpB, an undecaprenyl pyrophosphate phosphatase (2008), J. Biol. Chem., 283, 16573-16583.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | - |
Escherichia coli |  |
| diacylglycerol diphosphate | increases the activity with undecaprenyl diphosphate by about 400% | Escherichia coli | |
| phosphatidylglycerol | - |
Escherichia coli | |
| Phospholipids | - |
Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pgpB, overexpression of His-tagged enzyme in strain DH5alpha and C43 | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
diacylglycerol diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 0.096 | - |
farnesyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 0.53 | - |
undecaprenyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 1.7 | - |
phosphatidic acid | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 3.6 | - |
isopentenyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 3.9 | - |
diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral, PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location | Escherichia coli | 16020 | - |
| additional information | topological mapping, modelling, overview | Escherichia coli | - |
- |
| periplasm | PgpB contains six transmembrane segments, a large periplasmic loop, and the type 2 phosphatidic acid phosphatase signature residues at a periplasmic location | Escherichia coli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30158, mass spectrometry, x * 30000, SDS-PAGE | Escherichia coli |
| 30158 | - |
x * 30158, mass spectrometry, x * 30000, SDS-PAGE | Escherichia coli |
| 110000 | - |
recombinant His-tagged enzyme, gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| undecaprenyl diphosphate + H2O | Escherichia coli | the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview | undecaprenyl phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from membranes to homogeneity by ultracentrifugation, nickel affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
purified recombinant enzyme, substrate isopentenyl diphosphate | Escherichia coli |
| 3 | - |
purified recombinant enzyme, substrate undecaprenyl diphosphate | Escherichia coli |
| 6.7 | - |
purified recombinant enzyme, substrate phosphatidic acid | Escherichia coli |
| 300 | - |
purified recombinant enzyme, substrate farnesyl diphosphate | Escherichia coli |
| 310 | - |
purified recombinant enzyme, substrate diacylglycerol diphosphate | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylglycerol diphosphate + H2O | - |
Escherichia coli | diacylglycerol phosphate + phosphate | - |
? | |
| diphosphate + H2O | low activity | Escherichia coli | 2 phosphate | - |
? | |
| farnesyl diphosphate + H2O | - |
Escherichia coli | farnesyl phosphate + phosphate | - |
? | |
| isopentenyl diphosphate + H2O | low activity | Escherichia coli | isopentenyl phosphate + phosphate | - |
? | |
| additional information | substrate specificity, overview | Escherichia coli | ? | - |
? | |
| phosphatidic acid + H2O | low activity | Escherichia coli | ? | - |
? | |
| undecaprenyl diphosphate + H2O | low activity | Escherichia coli | undecaprenyl phosphate + phosphate | - |
? | |
| undecaprenyl diphosphate + H2O | the synthesis of the lipid carrier undecaprenyl phosphate, C55-P, requires the dephosphorylation of its precursor, undecaprenyl pyrophosphate, C55-PP, the dephosphorylation involves four integral membrane proteins, BacA, and three members of the type 2 phosphatidic acid phosphatase family, PgpB, YbjG, and YeiU, overview | Escherichia coli | undecaprenyl phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the enzyme exists as a monomer in n-dodecyl-beta-D-maltoside solution, topological mapping, overview | Escherichia coli |
| oligomer | x * 30158, mass spectrometry, x * 30000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme is a member of the type 2 phosphatidic acid phosphatase family | Escherichia coli |
| PgpB | - |
Escherichia coli |
| undecaprenyl pyrophosphate phosphatase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9 | - |
undecaprenyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 19 | - |
isopentenyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 48 | - |
diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 61 | - |
phosphatidic acid | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 280 | - |
diacylglycerol diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
| 290 | - |
farnesyl diphosphate | pH 7.5, 37°C, recombinant enzyme | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 50% of maximal activity at pH values other than pH 6.5-pH 7.5 | Escherichia coli |
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