Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | African swine fever virus |
Crystallization (Comment) | Organism |
---|---|
in complex with dUMP and Mg2+, sitting drop vapor diffusion method, using 0.16 M sodium chloride, 0.08 M HEPES pH 7.5, 0.02 M sodium citrate tribasic dihydrate, 17% (w/v) polyethylene glycol 3350 | African swine fever virus |
Protein Variants | Comment | Organism |
---|---|---|
D33A | the enzyme activity of the mutant is almost totally abolished | African swine fever virus |
D91A | the enzyme activity of the mutant is almost totally abolished | African swine fever virus |
F154A | the mutant exhibits approximately 10.5% of the activity of the wild type enzyme | African swine fever virus |
Q120A | the enzyme activity of the mutant is almost totally abolished | African swine fever virus |
R71A | the mutant exhibits approximately 9.6% of the activity of the wild type enzyme | African swine fever virus |
S72A | the mutant exhibits approximately 6.5% of the activity of the wild type enzyme | African swine fever virus |
Y94A | the mutant exhibits approximately 10% of the activity of the wild type enzyme | African swine fever virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | African swine fever virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
gel filtration | African swine fever virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | African swine fever virus | - |
dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
African swine fever virus | A0A2X0SE53 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex S200 gel filtration | African swine fever virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
African swine fever virus | dUMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | - |
African swine fever virus |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine 5'-triphosphate pyrophosphatase | - |
African swine fever virus |
dUTPase | - |
African swine fever virus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
84 | - |
melting temperature | African swine fever virus |