Literature summary for 3.6.1.23 extracted from

Zang, K.; Li, F.; Ma, Q.
The dUTPase of white spot syndrome virus assembles its active sites in a noncanonical manner (2018), J. Biol. Chem., 293, 1088-1099 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 30% (w/v) PEG 1500	Escherichia coli
sitting drop vapor diffusion method, using 30% (w/v) PEG 1500	white spot syndrome virus

Protein Variants

Protein Variants	Comment	Organism
D170G	the mutant shows 68% of wild type activity	white spot syndrome virus
D170L	the mutant shows 1.7% of wild type activity	white spot syndrome virus
D34N	the mutant shows 1.6% of wild type activity	white spot syndrome virus
D88N	inactive	white spot syndrome virus
DELTA134-153	the mutant shows 0.73% of wild type activity	white spot syndrome virus
DELTA161-170	inactive	white spot syndrome virus
DELTA170-171	the mutant shows 14% of wild type activity	white spot syndrome virus
F166Y	the mutant shows 5.5% of wild type activity	white spot syndrome virus
K150A	the mutant shows 54% of wild type activity	white spot syndrome virus
K150E	the mutant shows 49% of wild type activity	white spot syndrome virus
R116S	the mutant shows 5.2% of wild type activity	Escherichia coli
R158A	the mutant shows 28% of wild type activity	white spot syndrome virus
R158E	the mutant shows 23% of wild type activity	white spot syndrome virus
R158W	the mutant shows 22% of wild type activity	white spot syndrome virus
R161K	the mutant shows 0.23% of wild type activity	white spot syndrome virus
R24T	the mutant shows 3.6% of wild type activity	white spot syndrome virus
R24T/S115R	the mutant shows 1.8% of wild type activity	white spot syndrome virus
R24T/S115R/D170G	the mutant shows 17% of wild type activity	white spot syndrome virus
R71E	inactive	white spot syndrome virus
S115R	the mutant shows 13% of wild type activity	white spot syndrome virus
T22R/R116S	the mutant shows 2.5% of wild type activity	Escherichia coli
V160D	the mutant shows 85% of wild type activity	white spot syndrome virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.00093	-	dUTP	wild type enzyme, at pH 7.5 and 20°C	Escherichia coli
0.0017	-	dUTP	wild type enzyme, at pH 7.5 and 20°C	white spot syndrome virus
0.002	-	dUTP	mutant enzyme V160D, at pH 7.5 and 20°C	white spot syndrome virus
0.0021	-	dUTP	mutant enzyme D170G, at pH 7.5 and 20°C	white spot syndrome virus
0.0025	-	dUTP	mutant enzyme K150A, at pH 7.5 and 20°C	white spot syndrome virus
0.0026	-	dUTP	mutant enzyme K150E, at pH 7.5 and 20°C	white spot syndrome virus
0.0026	-	dUTP	mutant enzyme R158A, at pH 7.5 and 20°C	white spot syndrome virus
0.0026	-	dUTP	mutant enzyme R158E, at pH 7.5 and 20°C	white spot syndrome virus
0.0028	-	dUTP	mutant enzyme DELTA170-171, at pH 7.5 and 20°C	white spot syndrome virus
0.0029	-	dUTP	mutant enzyme R24T, at pH 7.5 and 20°C	white spot syndrome virus
0.0032	-	dUTP	mutant enzyme R158W, at pH 7.5 and 20°C	white spot syndrome virus
0.0042	-	dUTP	mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C	white spot syndrome virus
0.0045	-	dUTP	mutant enzyme S115R, at pH 7.5 and 20°C	white spot syndrome virus
0.005	-	dUTP	mutant enzyme F166Y, at pH 7.5 and 20°C	white spot syndrome virus
0.0052	-	dUTP	mutant enzyme R166S, at pH 7.5 and 20°C	Escherichia coli
0.0054	-	dUTP	mutant enzyme R161K, at pH 7.5 and 20°C	white spot syndrome virus
0.0067	-	dUTP	mutant enzyme R24T/S115R, at pH 7.5 and 20°C	white spot syndrome virus
0.0071	-	dUTP	mutant enzyme D170L, at pH 7.5 and 20°C	white spot syndrome virus
0.013	-	dUTP	mutant enzyme D34N, at pH 7.5 and 20°C	white spot syndrome virus
0.013	-	dUTP	mutant enzyme T22R/R116S, at pH 7.5 and 20°C	Escherichia coli
0.021	-	dUTP	mutant enzyme DELTA134-153, at pH 7.5 and 20°C	white spot syndrome virus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli
Mg2+	required	white spot syndrome virus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dUTP + H2O	Escherichia coli	-	dUMP + diphosphate	-	?
dUTP + H2O	white spot syndrome virus	-	dUMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
white spot syndrome virus	Q77J78	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
dUTP + H2O	-	Escherichia coli	dUMP + diphosphate	-	?
dUTP + H2O	-	white spot syndrome virus	dUMP + diphosphate	-	?
additional information	no activity with dUMP	Escherichia coli	?	-	-
additional information	no activity with dUMP	white spot syndrome virus	?	-	-

Subunits

Subunits	Comment	Organism
homotrimer	-	Escherichia coli
homotrimer	-	white spot syndrome virus

Synonyms

Synonyms	Comment	Organism
DUT	-	Escherichia coli
DUT	-	white spot syndrome virus
dUTPase	-	Escherichia coli
dUTPase	-	white spot syndrome virus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.053	-	dUTP	mutant enzyme R161K, at pH 7.5 and 20°C	white spot syndrome virus
0.45	-	dUTP	mutant enzyme R24T, at pH 7.5 and 20°C	white spot syndrome virus
0.51	-	dUTP	mutant enzyme R24T/S115R, at pH 7.5 and 20°C	white spot syndrome virus
0.53	-	dUTP	mutant enzyme D170L, at pH 7.5 and 20°C	white spot syndrome virus
0.67	-	dUTP	mutant enzyme DELTA134-153, at pH 7.5 and 20°C	white spot syndrome virus
0.92	-	dUTP	mutant enzyme D34N, at pH 7.5 and 20°C	white spot syndrome virus
1.2	-	dUTP	mutant enzyme F166Y, at pH 7.5 and 20°C	white spot syndrome virus
1.7	-	dUTP	mutant enzyme DELTA170-171, at pH 7.5 and 20°C	white spot syndrome virus
2.6	-	dUTP	mutant enzyme R158E, at pH 7.5 and 20°C	white spot syndrome virus
2.6	-	dUTP	mutant enzyme S115R, at pH 7.5 and 20°C	white spot syndrome virus
2.7	-	dUTP	mutant enzyme R166S, at pH 7.5 and 20°C	Escherichia coli
3.1	-	dUTP	mutant enzyme R158W, at pH 7.5 and 20°C	white spot syndrome virus
3.1	-	dUTP	mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C	white spot syndrome virus
3.2	-	dUTP	mutant enzyme R158A, at pH 7.5 and 20°C	white spot syndrome virus
3.3	-	dUTP	mutant enzyme T22R/R116S, at pH 7.5 and 20°C	Escherichia coli
5.5	-	dUTP	mutant enzyme K150E, at pH 7.5 and 20°C	white spot syndrome virus
5.9	-	dUTP	mutant enzyme K150A, at pH 7.5 and 20°C	white spot syndrome virus
6.2	-	dUTP	mutant enzyme D170G, at pH 7.5 and 20°C	white spot syndrome virus
7.4	-	dUTP	wild type enzyme, at pH 7.5 and 20°C	white spot syndrome virus
7.4	-	dUTP	mutant enzyme V160D, at pH 7.5 and 20°C	white spot syndrome virus
9.3	-	dUTP	wild type enzyme, at pH 7.5 and 20°C	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
9.8	-	dUTP	mutant enzyme R161K, at pH 7.5 and 20°C	white spot syndrome virus
32	-	dUTP	mutant enzyme DELTA134-153, at pH 7.5 and 20°C	white spot syndrome virus
71	-	dUTP	mutant enzyme D34N, at pH 7.5 and 20°C	white spot syndrome virus
74	-	dUTP	mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C	white spot syndrome virus
75	-	dUTP	mutant enzyme D170L, at pH 7.5 and 20°C	white spot syndrome virus
76	-	dUTP	mutant enzyme R24T/S115R, at pH 7.5 and 20°C	white spot syndrome virus
160	-	dUTP	mutant enzyme R24T, at pH 7.5 and 20°C	white spot syndrome virus
240	-	dUTP	mutant enzyme F166Y, at pH 7.5 and 20°C	white spot syndrome virus
250	-	dUTP	mutant enzyme T22R/R116S, at pH 7.5 and 20°C	Escherichia coli
520	-	dUTP	mutant enzyme R166S, at pH 7.5 and 20°C	Escherichia coli
580	-	dUTP	mutant enzyme S115R, at pH 7.5 and 20°C	white spot syndrome virus
610	-	dUTP	mutant enzyme DELTA170-171, at pH 7.5 and 20°C	white spot syndrome virus
970	-	dUTP	mutant enzyme R158W, at pH 7.5 and 20°C	white spot syndrome virus
1000	-	dUTP	mutant enzyme R158E, at pH 7.5 and 20°C	white spot syndrome virus
1200	-	dUTP	mutant enzyme R158A, at pH 7.5 and 20°C	white spot syndrome virus
2100	-	dUTP	mutant enzyme K150E, at pH 7.5 and 20°C	white spot syndrome virus
2400	-	dUTP	mutant enzyme K150A, at pH 7.5 and 20°C	white spot syndrome virus
3000	-	dUTP	mutant enzyme D170G, at pH 7.5 and 20°C	white spot syndrome virus
3700	-	dUTP	mutant enzyme V160D, at pH 7.5 and 20°C	white spot syndrome virus
4400	-	dUTP	wild type enzyme, at pH 7.5 and 20°C	white spot syndrome virus
10000	-	dUTP	wild type enzyme, at pH 7.5 and 20°C	Escherichia coli