Literature summary for 3.6.1.23 extracted from

Inoguchi, N.; Chaiseeda, K.; Yamanishi, M.; Kim, M.K.; Jang, Y.; Bajaj, M.; Chia, C.P.; Becker, D.F.; Moriyama, H.
Structural insights into the mechanism defining substrate affinity in Arabidopsis thaliana dUTPase the role of tryptophan 93 in ligand orientation (2015), BMC Res. Notes, 8, 784 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM103 (DE3) cells	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 50 mM Tris-HCl at pH 7.4 and 5 mM of the non-hydrolyzable ligand analog 2'-deoxyuridine 5'-[(alpha,beta)-imido]-triphosphate	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0004	-	dUTP	at pH 7.6 and 25°C	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	5 mM used in assay conditions	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dUTP + H2O	Arabidopsis thaliana	-	dUMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9STG6	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dUTP + H2O	-	Arabidopsis thaliana	dUMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	x-ray crystallography	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
deoxyuridine triphosphate nucleotidohydrolase	-	Arabidopsis thaliana
dUTPase	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)