Literature summary for 3.6.1.23 extracted from

Alam, M.S.; Moriyama, H.; Matsumoto, M.
Enzyme kinetics of dUTPase from the planarian Dugesia ryukyuensis (2019), BMC Res. Notes, 12, 163 .

Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Dugesia ryukyuensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	dUTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
0.0073	-	dUTP	in the presence of 5 mM Mn2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
0.0094	-	dATP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
0.077	-	dCTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
0.11	-	dTTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
0.21	-	dGTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dUTP + H2O	Dugesia ryukyuensis	-	dUMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Dugesia ryukyuensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Dugesia ryukyuensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dATP + H2O	-	Dugesia ryukyuensis	dAMP + diphosphate	-	?
dCTP + H2O	-	Dugesia ryukyuensis	dCMP + diphosphate	-	?
dGTP + H2O	-	Dugesia ryukyuensis	dGMP + diphosphate	-	?
dTTP + H2O	-	Dugesia ryukyuensis	dTMP + diphosphate	-	?
dUTP + H2O	-	Dugesia ryukyuensis	dUMP + diphosphate	-	?
dUTP + H2O	highest activity	Dugesia ryukyuensis	dUMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 16554, calculated from amino acid sequence	Dugesia ryukyuensis

Synonyms

Synonyms	Comment	Organism
DUT	-	Dugesia ryukyuensis
dUTPase	-	Dugesia ryukyuensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.4	-	dTTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
1.5	-	dATP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
1.6	-	dCTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
2.9	-	dGTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
34	-	dUTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
48	-	dUTP	in the presence of 5 mM Mn2+, at pH 8.2 and 25°C	Dugesia ryukyuensis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Dugesia ryukyuensis	calculated from amino acid sequence	-	5.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
13	-	dTTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
14	-	dGTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
16	-	dATP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
21	-	dCTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
6500	-	dUTP	in the presence of 5 mM Mn2+, at pH 8.2 and 25°C	Dugesia ryukyuensis
8500	-	dUTP	in the presence of 5 mM Mg2+, at pH 8.2 and 25°C	Dugesia ryukyuensis

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Release 2023.1 (January 2023)