Literature summary for 3.6.1.23 extracted from

Benedek, A.; Temesvary-Kis, F.; Khatanbaatar, T.; Leveles, I.; Suranyi, E.V.; Szabo, J.E.; Wunderlich, L.; Vertessy, B.G.
The role of a key amino acid position in species-specific proteinaceous dUTPase inhibition (2019), Biomolecules, 9, 221 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli (DE3) Rosetta cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
Q93H mutant in complex with a non-hydrolysable substrate analogue, alpha,beta-imido-dUTP, hanging drop vapor diffusion method, using 0.1 M Tris, 18-33.75 % polyethylene glycol 3350, 400 mM NaAc, pH 7.5	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
Q93H	the mutant enzyme is significantly inhibited by staphylococcal repressor StlSaPIbov1 protein (Ki 0.00000483 mM)	Escherichia coli
Q93R	the mutant enzyme is significantly inhibited by staphylococcal repressor StlSaPIbov1 protein (Ki 0.0000059 mM)	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the wild type enzyme is not inhibited by staphylococcal repressor StlSaPIbov1 protein	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dUTP + H2O	Escherichia coli	-	dUMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P06968	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dUTP + H2O	-	Escherichia coli	dUMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
DUT	-	Escherichia coli
dUTPase	-	Escherichia coli

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Release 2023.1 (January 2023)