Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(2-amido-4-trityloxybutyl)uracil | - |
Plasmodium falciparum | |
1-(2-dimethylamido-4-trityloxybutyl)uracil | - |
Plasmodium falciparum | |
1-(2-morpholinamido-4-trityloxybutyl)uracil | - |
Plasmodium falciparum | |
1-[2-(acetamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | - |
Plasmodium falciparum | |
1-[2-(acetamido)-4-(triphenylmethyl)aminobutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(carbobenzoxymethyl)-4-(triphenylmethyl)aminobutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(carboethoxyethylamido)-4-trityloxybutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(carboxy)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | - |
Plasmodium falciparum | |
1-[2-(carboxy)-4-(triphenylmethyl)aminobutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(carboxyethylamido)-4-trityloxybutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(diethylamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | - |
Plasmodium falciparum | |
1-[2-(ethylamido)-4-(tert-butyldiphenylsilyloxy) butyl]uracil | - |
Plasmodium falciparum | |
1-[2-(hydroxyethylamido)-4-trityloxybutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(methoxyethylamido)-4-trityloxybutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(methylamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | - |
Plasmodium falciparum | |
1-[2-(N,N-dimethylaminoethylamido)-4-trityloxybutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(N-benzyloxycarbonylpiperazinamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | - |
Plasmodium falciparum | |
1-[2-(N-methylpiperazine)amido-4-trityloxybutyl]uracil | - |
Plasmodium falciparum | |
1-[2-(tert-butyldiphenylsilyloxyethylamido)-4-trityloxybutyl]uracil | - |
Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Plasmodium falciparum | - |
dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8II92 | - |
- |
Purification (Comment) | Organism |
---|---|
phosphocellulose column chromatography and Superdex 200 gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Plasmodium falciparum | dUMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine-5'-triphosphate nucleotidohydrolase | - |
Plasmodium falciparum |
dUTPase | - |
Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
1-[2-(carboxyethylamido)-4-trityloxybutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0004 | - |
1-[2-(hydroxyethylamido)-4-trityloxybutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0005 | - |
1-[2-(carboethoxyethylamido)-4-trityloxybutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0013 | - |
1-(2-amido-4-trityloxybutyl)uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0014 | - |
1-[2-(methoxyethylamido)-4-trityloxybutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0016 | - |
1-[2-(carboxy)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0021 | - |
1-(2-morpholinamido-4-trityloxybutyl)uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0027 | - |
1-(2-dimethylamido-4-trityloxybutyl)uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0036 | - |
1-[2-(methylamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.005 | - |
1-[2-(acetamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0055 | - |
1-[2-(acetamido)-4-(triphenylmethyl)aminobutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0056 | - |
1-[2-(N-methylpiperazine)amido-4-trityloxybutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0057 | - |
1-[2-(ethylamido)-4-(tert-butyldiphenylsilyloxy) butyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0065 | - |
1-[2-(carbobenzoxymethyl)-4-(triphenylmethyl)aminobutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0072 | - |
1-[2-(carboxy)-4-(triphenylmethyl)aminobutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.0078 | - |
1-[2-(N,N-dimethylaminoethylamido)-4-trityloxybutyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.01 | - |
1-[2-(tert-butyldiphenylsilyloxyethylamido)-4-trityloxybutyl]uracil | Ki above 0.01 mM, pH and temperature not specified in the publication | Plasmodium falciparum | |
0.02 | - |
1-[2-(diethylamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum | |
0.098 | - |
1-[2-(N-benzyloxycarbonylpiperazinamido)-4-(tert-butyldiphenylsilyloxy)butyl]uracil | pH and temperature not specified in the publication | Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in nucleotide metabolism that acts as first line of defence against uracil incorporation into DNA | Plasmodium falciparum |