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Literature summary for 3.6.1.23 extracted from

  • Castillo-Acosta, V.M.; Estevez, A.M.; Vidal, A.E.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
    Depletion of dimeric all-alpha dUTPase induces DNA strand breaks and impairs cell cycle progression in Trypanosoma brucei (2008), Int. J. Biochem. Cell Biol., 40, 2901-2913.
    View publication on PubMed
Search for more literature for 3.6.1.23

Cloned(Commentary)

Cloned (Comment) Organism
gene DUT, recombinant expression Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
additional information down-regulation of the enzyme activity by RNAi greatly reduces cell proliferation and increases the intracellular levels of dUTP. Defects in growth can be partially reverted by the addition of exogenous thymidine. dUTPase-depleted cells present hypersensitivity to methotrexate, a drug that increases the intracellular pools of dUTP, and enhanced uracil-DNA glycosylase activity, the first step in base excision repair, overview Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00179
-
 dUTP pH 7.4, 37°C, recombinant enzyme Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information analysis of subcellular localization of dUTPase, no localization in the mitochondrion or kinetoplast Trypanosoma brucei
-
-
nucleus
-
 Trypanosoma brucei 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dUTP + H2O Trypanosoma brucei dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA dUMP + diphosphate
-
 ?
dUTP + H2O Trypanosoma brucei 427 dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA dUMP + diphosphate
-
 ?
additional information Trypanosoma brucei procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation ?
-
 ?
additional information Trypanosoma brucei 427 procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
 gene DUT with T49 and E248
-
Trypanosoma brucei 427
-
 gene DUT with T49 and E248
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme is expressed in different life stages. Trypanosoma brucei brucei bloodstream form cell line S16 and the procyclic cell line 449 Trypanosoma brucei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
 Trypanosoma brucei dUMP + diphosphate
-
 ?
dUTP + H2O dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA Trypanosoma brucei dUMP + diphosphate
-
 ?
dUTP + H2O
-
 Trypanosoma brucei 427 dUMP + diphosphate
-
 ?
dUTP + H2O dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA Trypanosoma brucei 427 dUMP + diphosphate
-
 ?
additional information procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation Trypanosoma brucei ?
-
 ?
additional information procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation Trypanosoma brucei 427 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Trypanosoma brucei

Synonyms

Synonyms Comment Organism
deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Trypanosoma brucei
dUTPase
-
 Trypanosoma brucei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.7
-
 dUTP pH 7.4, 37°C, recombinant enzyme Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Trypanosoma brucei