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Literature summary for 3.6.1.23 extracted from
- Characterization of a dUTPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in polymerase chain reaction amplification (2007), Mar. Biotechnol., 9, 450-458.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, His-tagged enzyme expression in Escherichia coli | Thermococcus onnurineus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | 38% inhibition at 0.01% | Thermococcus onnurineus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzymatic activity of the dUTPase is largely unaffected by variations in MgCl2, KCl, (NH4)2SO4, and Triton X-100 concentrations | Thermococcus onnurineus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17700 | - |
2 * 17700, about, sequence calculation | Thermococcus onnurineus |
| 43000 | - |
recombinant enzyme, gel filtration | Thermococcus onnurineus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCTP + H2O | Thermococcus onnurineus | - |
dCMP + diphosphate | - |
? |  |
| dUTP + H2O | Thermococcus onnurineus | the enzyme plays an essential role in nucleotide biosynthesis, dUMP is required for de novo synthesis of dTTP. The enzyme maintains low cellular ratios of dUTP:dTTP, thus preventing the misincorporation of uracil into chromosomal DNA | dUMP + diphosphate | - |
? | |
| additional information | Thermococcus onnurineus | the enzyme is essential for survival | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus onnurineus | A7J1X3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by cobalt chelating affinity chromatography and gel filtration | Thermococcus onnurineus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCTP + H2O | - |
Thermococcus onnurineus | dCMP + diphosphate | - |
? | |
| dCTP + H2O | about 10fold lower activity with dCTP compared to dUTP | Thermococcus onnurineus | dCMP + diphosphate | - |
? | |
| dUTP + H2O | preferred substrate | Thermococcus onnurineus | dUMP + diphosphate | - |
? | |
| dUTP + H2O | the enzyme plays an essential role in nucleotide biosynthesis, dUMP is required for de novo synthesis of dTTP. The enzyme maintains low cellular ratios of dUTP:dTTP, thus preventing the misincorporation of uracil into chromosomal DNA | Thermococcus onnurineus | dUMP + diphosphate | - |
? | |
| additional information | the enzyme is essential for survival | Thermococcus onnurineus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 17700, about, sequence calculation | Thermococcus onnurineus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| deoxyuridine-triphosphate pyrophosphatase | - |
Thermococcus onnurineus |
| dUTPase | - |
Thermococcus onnurineus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermococcus onnurineus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 95 | temperature profile | Thermococcus onnurineus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
purified recombinant enzyme, half-life is 170 min | Thermococcus onnurineus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thermococcus onnurineus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.8 | pH profile | Thermococcus onnurineus |
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