Application | Comment | Organism |
---|---|---|
drug development | inhibitor development against the enzyme can be useful in antiviral and anticancer therapy | Drosophila melanogaster |
drug development | inhibitor development against the enzyme can be useful in antiviral and anticancer therapy | Escherichia coli |
pharmacology | the enzyme is a chemotherapeutic target | Drosophila melanogaster |
pharmacology | the enzyme is a chemotherapeutic target | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme possesses a specific tryptic cleavage site Arg132-Ile133 20 A far from the active site of the enzyme, the enzyme is protected against tryptic digestion by binding alpha,beta-imino-dUTP which induces an allosteric conformational change within the central threefold channel of the homotrimer | Drosophila melanogaster | |
additional information | binding of alpha,beta-imino-dUTP does not induce allosteric conformational changes and does not protect the enzyme against tryptic digestion | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of dUTP binding | Drosophila melanogaster | |
additional information | - |
additional information | kinetics of dUTP binding | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binding as dUTP-Mg2+ at the C-terminus induces the closure of the C-terminal arm | Drosophila melanogaster | |
Mg2+ | binding as dUTP-Mg2+ at the C-terminus induces the closure of the C-terminal arm which opens up again after hydrolysis to expel the product | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | Drosophila melanogaster | the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis | dUMP + diphosphate | - |
? | |
dUTP + H2O | Escherichia coli | the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis | dUMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Drosophila melanogaster |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dUTP + H2O = dUMP + diphosphate | structural and catalytic mechanism involving Mg2+ | Drosophila melanogaster | |
dUTP + H2O = dUMP + diphosphate | structural and catalytic mechanism involving Mg2+ | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis | Drosophila melanogaster | dUMP + diphosphate | - |
? | |
dUTP + H2O | the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis | Escherichia coli | dUMP + diphosphate | - |
? | |
dUTP + H2O | the enzyme is highly specific for dUTP | Drosophila melanogaster | dUMP + diphosphate | - |
? | |
dUTP + H2O | the enzyme is highly specific for dUTP | Escherichia coli | dUMP + diphosphate | - |
? | |
additional information | comparison of the Drosophila melanogaster and the Escherichia coli enzymes | Drosophila melanogaster | ? | - |
? | |
additional information | comparison of the Drosophila melanogaster and the Escherichia coli enzymes | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | each subunit contains an active site | Escherichia coli |
trimer | each subunit contains an active site, alpha,beta-imino-dUTP which induces an allosteric conformational change within the central threefold channel of the homotrimer | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
dUTPase | - |
Drosophila melanogaster |
dUTPase | - |
Escherichia coli |