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Literature summary for 3.6.1.23 extracted from
- Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA (1978), J. Biol. Chem., 253, 3305-3312.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | can reduce uracil incorporation into DNA | Escherichia coli |
General Stability
| General Stability | Organism |
|---|---|
| stable to freezing and thawing | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 2 mM, complete inhibition | Escherichia coli |  |
| Mg2+ | complete inhibition by extensive dialysis against 0.0125 M MgCl2 | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
dUTP | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | no requirement for Mg2+ | Escherichia coli | |
| Zn2+ | appears to be essential for activity | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
4 * 16000, SDS-PAGE | Escherichia coli |
| 64500 | - |
gel filtration, sucrose density gradient centrifugation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 5 months | Escherichia coli |
| 0°C, stable for at least 3 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dUTP + H2O | - |
Escherichia coli | dUMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 16000, SDS-PAGE | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 6.1 | 8 | pH 5.0: no activity, pH 6.1-7.5: optimum, pH 8.0: about 60% of maximal activity | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38.3 | - |
dUTP | at 30°C | Escherichia coli | |
| 58.3 | - |
dUTP | at 35°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | 7.5 | - |
Escherichia coli |
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