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Literature summary for 3.6.1.22 extracted from

  • Wang, X.D.; Gu, J.; Wang, T.; Bi, L.J.; Zhang, Z.P.; Cui, Z.Q.; Wei, H.P.; Deng, J.Y.; Zhang, X.E.
    Comparative analysis of mycobacterial NADH pyrophosphatase isoforms reveals a novel mechanism for isoniazid and ethionamide inactivation (2011), Mol. Microbiol., 82, 1375-1391.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Mycobacterium tuberculosis
expressed in Escherichia coli BL21 (DE3) cells and in Mycobacterium smegmatis strain mc2155 Mycobacterium tuberculosis variant bovis

Protein Variants

Protein Variants Comment Organism
P237Q the mutation prevents dimer formation, and results in a loss of enzymatic activity Mycobacterium tuberculosis variant bovis
P237Q the mutation prevents dimer formation, and results in a loss of enzymatic activity Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ no activity in the presence of Ca2+ Mycobacterium tuberculosis
Ca2+ no activity in the presence of Ca2+ Mycobacterium tuberculosis variant bovis
Cu2+ no activity in the presence of Cu2+ Mycobacterium tuberculosis
Cu2+ no activity in the presence of Cu2+ Mycobacterium tuberculosis variant bovis
Fe2+ no activity in the presence of Fe2+ Mycobacterium tuberculosis
Fe3+ no activity in the presence of Fe3+ Mycobacterium tuberculosis
Fe3+ no activity in the presence of Fe3+ Mycobacterium tuberculosis variant bovis
Mg2+ no activity in the presence of Mg2+ Mycobacterium tuberculosis
Zn2+ no activity in the presence of Zn2+ Mycobacterium tuberculosis
Zn2+ no activity in the presence of Zn2+ Mycobacterium tuberculosis variant bovis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme shows some activity on NADH in the presence of Fe2+ Mycobacterium tuberculosis variant bovis
Mg2+ Mg2+ has highest stimulating effect Mycobacterium tuberculosis variant bovis
Mn2+ Mn2+ has highest stimulating effect Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Mycobacterium tuberculosis variant bovis
50000
-
gel filtration Mycobacterium tuberculosis
51000
-
1 * 51000, purified recombinant NudC (fused with Trx tag), calculated from amino acid sequence Mycobacterium tuberculosis
80000
-
gel filtration Mycobacterium tuberculosis variant bovis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIX5
-
-
Mycobacterium tuberculosis H37Rv P9WIX5
-
-
Mycobacterium tuberculosis variant bovis C1AGW8
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis variant bovis
nickel affinity column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethionamide-NAD+ + H2O
-
Mycobacterium tuberculosis variant bovis ethionamide-NMNH + AMP
-
?
isoniazid-NAD+ + H2O
-
Mycobacterium tuberculosis variant bovis isoniazid-NMNH + AMP
-
?
NAD+ + H2O weak substrate Mycobacterium tuberculosis variant bovis NMN + AMP
-
?
NAD+ + H2O weak substrate Mycobacterium tuberculosis NMN + AMP
-
?
NAD+ + H2O weak substrate Mycobacterium tuberculosis H37Rv NMN + AMP
-
?
NADH + H2O highest activity with NADH Mycobacterium tuberculosis variant bovis NMNH + AMP
-
?
NADH + H2O highest activity with NADH Mycobacterium tuberculosis NMNH + AMP
-
?
NADH + H2O highest activity with NADH Mycobacterium tuberculosis H37Rv NMNH + AMP
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 40000, SDS-PAGE Mycobacterium tuberculosis variant bovis
monomer 1 * 50000, SDS-PAGE Mycobacterium tuberculosis
monomer 1 * 51000, purified recombinant NudC (fused with Trx tag), calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
NADH pyrophosphatase
-
Mycobacterium tuberculosis variant bovis
NADH pyrophosphatase
-
Mycobacterium tuberculosis
NudC
-
Mycobacterium tuberculosis variant bovis
NudC
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Mycobacterium tuberculosis variant bovis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 45 enzyme activity rapidly decreases below 35°C or above 45°C Mycobacterium tuberculosis variant bovis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
enzyme activity rapidly decreases below pH 7.5 Mycobacterium tuberculosis variant bovis

General Information

General Information Comment Organism
malfunction deletion of the NADH pyrophosphatase gene nudC results in increased susceptibility to isoniazid and ethionamide Mycobacterium tuberculosis variant bovis
physiological function NADH pyrophosphatase NudC plays an important role in the inactivation of isoniazid and ethionamide Mycobacterium tuberculosis variant bovis