Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Mycobacterium tuberculosis |
expressed in Escherichia coli BL21 (DE3) cells and in Mycobacterium smegmatis strain mc2155 | Mycobacterium tuberculosis variant bovis |
Protein Variants | Comment | Organism |
---|---|---|
P237Q | the mutation prevents dimer formation, and results in a loss of enzymatic activity | Mycobacterium tuberculosis variant bovis |
P237Q | the mutation prevents dimer formation, and results in a loss of enzymatic activity | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no activity in the presence of Ca2+ | Mycobacterium tuberculosis | |
Ca2+ | no activity in the presence of Ca2+ | Mycobacterium tuberculosis variant bovis | |
Cu2+ | no activity in the presence of Cu2+ | Mycobacterium tuberculosis | |
Cu2+ | no activity in the presence of Cu2+ | Mycobacterium tuberculosis variant bovis | |
Fe2+ | no activity in the presence of Fe2+ | Mycobacterium tuberculosis | |
Fe3+ | no activity in the presence of Fe3+ | Mycobacterium tuberculosis | |
Fe3+ | no activity in the presence of Fe3+ | Mycobacterium tuberculosis variant bovis | |
Mg2+ | no activity in the presence of Mg2+ | Mycobacterium tuberculosis | |
Zn2+ | no activity in the presence of Zn2+ | Mycobacterium tuberculosis | |
Zn2+ | no activity in the presence of Zn2+ | Mycobacterium tuberculosis variant bovis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme shows some activity on NADH in the presence of Fe2+ | Mycobacterium tuberculosis variant bovis | |
Mg2+ | Mg2+ has highest stimulating effect | Mycobacterium tuberculosis variant bovis | |
Mn2+ | Mn2+ has highest stimulating effect | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
50000 | - |
gel filtration | Mycobacterium tuberculosis |
51000 | - |
1 * 51000, purified recombinant NudC (fused with Trx tag), calculated from amino acid sequence | Mycobacterium tuberculosis |
80000 | - |
gel filtration | Mycobacterium tuberculosis variant bovis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WIX5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WIX5 | - |
- |
Mycobacterium tuberculosis variant bovis | C1AGW8 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis variant bovis |
nickel affinity column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethionamide-NAD+ + H2O | - |
Mycobacterium tuberculosis variant bovis | ethionamide-NMNH + AMP | - |
? | |
isoniazid-NAD+ + H2O | - |
Mycobacterium tuberculosis variant bovis | isoniazid-NMNH + AMP | - |
? | |
NAD+ + H2O | weak substrate | Mycobacterium tuberculosis variant bovis | NMN + AMP | - |
? | |
NAD+ + H2O | weak substrate | Mycobacterium tuberculosis | NMN + AMP | - |
? | |
NAD+ + H2O | weak substrate | Mycobacterium tuberculosis H37Rv | NMN + AMP | - |
? | |
NADH + H2O | highest activity with NADH | Mycobacterium tuberculosis variant bovis | NMNH + AMP | - |
? | |
NADH + H2O | highest activity with NADH | Mycobacterium tuberculosis | NMNH + AMP | - |
? | |
NADH + H2O | highest activity with NADH | Mycobacterium tuberculosis H37Rv | NMNH + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
monomer | 1 * 50000, SDS-PAGE | Mycobacterium tuberculosis |
monomer | 1 * 51000, purified recombinant NudC (fused with Trx tag), calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
NADH pyrophosphatase | - |
Mycobacterium tuberculosis variant bovis |
NADH pyrophosphatase | - |
Mycobacterium tuberculosis |
NudC | - |
Mycobacterium tuberculosis variant bovis |
NudC | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Mycobacterium tuberculosis variant bovis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | enzyme activity rapidly decreases below 35°C or above 45°C | Mycobacterium tuberculosis variant bovis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
enzyme activity rapidly decreases below pH 7.5 | Mycobacterium tuberculosis variant bovis |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the NADH pyrophosphatase gene nudC results in increased susceptibility to isoniazid and ethionamide | Mycobacterium tuberculosis variant bovis |
physiological function | NADH pyrophosphatase NudC plays an important role in the inactivation of isoniazid and ethionamide | Mycobacterium tuberculosis variant bovis |