BRENDA - Enzyme Database show
show all sequences of 3.6.1.22

Cloning and characterization of the NADH pyrophospatases from Caenorhabditis elegans and Saccharomyces cerevisiae, members of a nudix hydrolase subfamily

Xu, W.; Dunn, C.A.; Bessman, M.J.; Biochem. Biophys. Res. Commun. 273, 753-758 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Caenorhabditis elegans
expressed in Escherichia coli
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
in presence of Mg2+ complete inhibition
Caenorhabditis elegans
Ca2+
in presence of Mg2+ complete inhibition
Saccharomyces cerevisiae
Zn2+
in presence of Mg2+ complete inhibition
Caenorhabditis elegans
Zn2+
in presence of Mg2+ complete inhibition
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4
-
NADH
50 mM Tris-HCl, pH 8.5, 5 mM MgCl2
Caenorhabditis elegans
1.6
-
NADH
50 mM Tris-HCl, pH 7.5, 5 mM MgCl2
Saccharomyces cerevisiae
6.6
-
NAD+
50 mM Tris-HCl, pH 8.5, 5 mM MgCl2
Caenorhabditis elegans
6.6
-
NAD+
50 mM Tris-HCl, pH 7.5, 5 mM MgCl2
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
strong activation
Caenorhabditis elegans
Mg2+
strong activation
Escherichia coli
Mg2+
strong activation
Saccharomyces cerevisiae
Mn2+
strong activation
Caenorhabditis elegans
Mn2+
strong activation
Escherichia coli
Mn2+
strong activation
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39100
-
homodimer, 2 * 39100
Caenorhabditis elegans
43500
-
homodimer, 2 * 43500
Saccharomyces cerevisiae
78200
-
two subunits of 39100 Da found
Caenorhabditis elegans
87000
-
two subunits of 43500 Da found
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NADH + H2O
Escherichia coli
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Escherichia coli
ir
NADH + H2O
Saccharomyces cerevisiae
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Saccharomyces cerevisiae
ir
NADH + H2O
Caenorhabditis elegans
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Caenorhabditis elegans
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caenorhabditis elegans
-
-
-
Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
purification of gene product from Escherichia coli
Caenorhabditis elegans
purification of gene product from Escherichia coli
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADPribose + H2O
-
209959
Escherichia coli
?
-
-
-
?
ADPribose + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
ADPribose + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
AppA + H2O
-
209959
Escherichia coli
?
-
-
-
?
AppA + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
AppA + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
dATP + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
NAD+ + H2O
-
209959
Escherichia coli
NMN + AMP
-
-
-
ir
NAD+ + H2O
-
209959
Saccharomyces cerevisiae
NMN + AMP
-
-
-
ir
NAD+ + H2O
-
209959
Caenorhabditis elegans
NMN + AMP
-
-
-
ir
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Escherichia coli
NMNH + AMP
-
209959
Escherichia coli
ir
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Saccharomyces cerevisiae
NMNH + AMP
-
209959
Saccharomyces cerevisiae
ir
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Caenorhabditis elegans
NMNH + AMP
-
209959
Caenorhabditis elegans
ir
NADH + H2O
-
209959
Escherichia coli
AMP + NMNH
-
209959
Escherichia coli
?
NADH + H2O
-
209959
Saccharomyces cerevisiae
AMP + NMNH
-
209959
Saccharomyces cerevisiae
?
NADH + H2O
-
209959
Caenorhabditis elegans
AMP + NMNH
-
209959
Caenorhabditis elegans
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Escherichia coli
?
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
homodimer, 2 * 39100
Caenorhabditis elegans
dimer
-
Escherichia coli
dimer
homodimer, 2 * 43500
Saccharomyces cerevisiae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.000167
-
NAD+
-
Caenorhabditis elegans
0.000167
-
NAD+
-
Saccharomyces cerevisiae
0.00117
-
NADH
-
Caenorhabditis elegans
0.0025
-
NADH
-
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Saccharomyces cerevisiae
8.5
-
-
Caenorhabditis elegans
8.5
-
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Caenorhabditis elegans
expressed in Escherichia coli
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
in presence of Mg2+ complete inhibition
Caenorhabditis elegans
Ca2+
in presence of Mg2+ complete inhibition
Saccharomyces cerevisiae
Zn2+
in presence of Mg2+ complete inhibition
Caenorhabditis elegans
Zn2+
in presence of Mg2+ complete inhibition
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4
-
NADH
50 mM Tris-HCl, pH 8.5, 5 mM MgCl2
Caenorhabditis elegans
1.6
-
NADH
50 mM Tris-HCl, pH 7.5, 5 mM MgCl2
Saccharomyces cerevisiae
6.6
-
NAD+
50 mM Tris-HCl, pH 8.5, 5 mM MgCl2
Caenorhabditis elegans
6.6
-
NAD+
50 mM Tris-HCl, pH 7.5, 5 mM MgCl2
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
strong activation
Caenorhabditis elegans
Mg2+
strong activation
Escherichia coli
Mg2+
strong activation
Saccharomyces cerevisiae
Mn2+
strong activation
Caenorhabditis elegans
Mn2+
strong activation
Escherichia coli
Mn2+
strong activation
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39100
-
homodimer, 2 * 39100
Caenorhabditis elegans
43500
-
homodimer, 2 * 43500
Saccharomyces cerevisiae
78200
-
two subunits of 39100 Da found
Caenorhabditis elegans
87000
-
two subunits of 43500 Da found
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NADH + H2O
Escherichia coli
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Escherichia coli
ir
NADH + H2O
Saccharomyces cerevisiae
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Saccharomyces cerevisiae
ir
NADH + H2O
Caenorhabditis elegans
possible function in regulation of NAD+/NADH-ratio
NMNH + AMP
-
Caenorhabditis elegans
ir
Purification (Commentary) (protein specific)
Commentary
Organism
purification of gene product from Escherichia coli
Caenorhabditis elegans
purification of gene product from Escherichia coli
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADPribose + H2O
-
209959
Escherichia coli
?
-
-
-
?
ADPribose + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
ADPribose + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
AppA + H2O
-
209959
Escherichia coli
?
-
-
-
?
AppA + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
AppA + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
dATP + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
NAD+ + H2O
-
209959
Escherichia coli
NMN + AMP
-
-
-
ir
NAD+ + H2O
-
209959
Saccharomyces cerevisiae
NMN + AMP
-
-
-
ir
NAD+ + H2O
-
209959
Caenorhabditis elegans
NMN + AMP
-
-
-
ir
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Escherichia coli
NMNH + AMP
-
209959
Escherichia coli
ir
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Saccharomyces cerevisiae
NMNH + AMP
-
209959
Saccharomyces cerevisiae
ir
NADH + H2O
possible function in regulation of NAD+/NADH-ratio
209959
Caenorhabditis elegans
NMNH + AMP
-
209959
Caenorhabditis elegans
ir
NADH + H2O
-
209959
Escherichia coli
AMP + NMNH
-
209959
Escherichia coli
?
NADH + H2O
-
209959
Saccharomyces cerevisiae
AMP + NMNH
-
209959
Saccharomyces cerevisiae
?
NADH + H2O
-
209959
Caenorhabditis elegans
AMP + NMNH
-
209959
Caenorhabditis elegans
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Escherichia coli
?
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Saccharomyces cerevisiae
?
-
-
-
?
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
209959
Caenorhabditis elegans
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
homodimer, 2 * 39100
Caenorhabditis elegans
dimer
-
Escherichia coli
dimer
homodimer, 2 * 43500
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.000167
-
NAD+
-
Caenorhabditis elegans
0.000167
-
NAD+
-
Saccharomyces cerevisiae
0.00117
-
NADH
-
Caenorhabditis elegans
0.0025
-
NADH
-
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Saccharomyces cerevisiae
8.5
-
-
Caenorhabditis elegans
8.5
-
-
Escherichia coli
Other publictions for EC 3.6.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733748
Wang
Identification of UshA as a ma ...
Escherichia coli, Escherichia coli DH10B
Enzyme Microb. Technol.
58-59
75-79
2014
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
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3
-
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3
-
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-
-
-
-
-
-
-
-
4
-
-
-
-
3
-
-
-
-
-
1
1
-
3
3
720498
Wang
Comparative analysis of mycoba ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycobacterium tuberculosis variant bovis
Mol. Microbiol.
82
1375-1391
2011
-
-
2
-
2
-
10
-
-
3
4
-
-
8
-
-
2
-
-
-
-
-
8
2
1
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
10
-
-
-
3
4
-
-
-
-
2
-
-
-
-
8
2
1
1
-
-
1
-
-
-
-
2
2
-
-
-
670632
Jambunathan
Analysis of Arabidopsis growth ...
Arabidopsis sp.
Planta
224
1-11
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
1
-
-
-
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1
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1
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1
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1
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-
-
-
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-
668217
Magni
Enzymology of NAD+ homeostasis ...
Homo sapiens
Cell. Mol. Life Sci.
61
19-34
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654332
AbdelRaheim
The NADH diphosphatase encoded ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
388
18-24
2001
-
-
1
-
-
-
-
3
1
-
-
1
-
1
-
1
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
1
-
-
1
-
-
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655747
Schmidt-Brauns
Is a NAD pyrophosphatase activ ...
Haemophilus influenzae
Int. J. Med. Microbiol.
291
219-225
2001
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
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-
-
2
-
-
1
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1
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2
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1
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-
-
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-
656433
Bortell
Nicotinamide adenine dinucleot ...
Rattus norvegicus
J. Immunol.
167
2049-2059
2001
-
1
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
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1
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1
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1
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1
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1
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-
209959
Xu
Cloning and characterization o ...
Caenorhabditis elegans, Escherichia coli, Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
273
753-758
2000
-
-
2
-
-
-
4
4
-
6
4
3
-
6
-
-
2
-
-
-
-
-
19
3
-
-
-
4
3
-
-
-
-
-
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-
-
2
-
-
-
-
-
4
-
4
-
6
4
3
-
-
-
2
-
-
-
-
19
3
-
-
-
4
3
-
-
-
-
-
-
-
-
-
209958
Hagen
Detection and identification o ...
Rattus norvegicus
Biochim. Biophys. Acta
1340
7-12
1997
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
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-
-
1
2
-
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1
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1
1
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1
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
209957
Frick
Cloning, purification, and pro ...
Escherichia coli
J. Biol. Chem.
270
1529-1534
1995
-
-
1
-
-
-
1
6
-
5
2
1
-
2
-
-
1
-
-
-
-
-
13
1
-
-
-
1
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
6
-
5
2
1
-
-
-
1
-
-
-
-
13
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
171771
Wagner
The pyridine-nucleotide cycle ...
Nicotiana tabacum
Planta
167
226-232
1986
-
-
-
-
-
-
-
1
-
-
-
1
-
4
-
-
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-
1
1
1
3
-
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1
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1
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1
1
1
3
-
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-
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-
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-
-
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-
-
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-
171772
Wagner
Regulation in tobacco callus o ...
Nicotiana tabacum
Planta
168
408-413
1986
-
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1
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1
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1
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2
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209951
Davies
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Jeck
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209949
Nakajima
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Anderson
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