Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',5'-ADP | 44% inhibition at concentration of 0.133 mM | Proteus vulgaris | |
ADP | 21% inhibition at concentration of 0.133 mM | Proteus vulgaris | |
ATP | 22% inhibition at concentration of 0.066 mM | Proteus vulgaris | |
Ca2+ | 20% inhibition at concentration of 0.33 mM | Proteus vulgaris | |
Co2+ | 40% inhibition of the purified enzyme at concentration of 0.33 mM, activity in crude extract not affected | Proteus vulgaris | |
CoA | 85% inhibition at concentration of 0.133 mM | Proteus vulgaris | |
CTP | 55% inhibition at concentration of 0.066 mM | Proteus vulgaris | |
DNA | strong inhibition by denaturated DNA, slight inhibition by native DNA | Proteus vulgaris | |
EDTA | 87% inhibition, reactivation by Mn2+ or Co2+ | Proteus vulgaris | |
FAD | 35% inhibition at concentration of 0.133 mM | Proteus vulgaris | |
GTP | 53% inhibition at concentration of 0.066 mM | Proteus vulgaris | |
RNA | strong inhibition | Proteus vulgaris | |
UDP | 32% inhibition at concentration of 0.133 mM | Proteus vulgaris | |
UTP | 36% inhibition at concentration of 0.066 mM | Proteus vulgaris | |
Zn2+ | 70% inhibition at concentration of 0.33 mM | Proteus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADP+ | Tris-HCl, pH 7.0 | Proteus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | reactivation of EDTA-treated enzyme | Proteus vulgaris | |
Mg2+ | slight activation at concentration of 1.0 mM | Proteus vulgaris | |
Mn2+ | slight activation at concentration of 1.0 mM, reactivation of EDTA-treated enzyme | Proteus vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADP+ + H2O | Proteus vulgaris | possible function in regulation of NADP-level | NMN + adenosine 3',5'-diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Proteus vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0008 | - |
crude extract | Proteus vulgaris |
0.78 | - |
purified enzyme with 1 mM MnCl2 | Proteus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Proteus vulgaris | AMP + phosphate | - |
? | |
AMP + H2O | - |
Proteus vulgaris | adenosine + phosphate | - |
? | |
NAD+ + H2O | - |
Proteus vulgaris | NMN + AMP | - |
ir | |
NADH + H2O | - |
Proteus vulgaris | AMP + NMNH | - |
? | |
NADP+ + H2O | - |
Proteus vulgaris | NMN + adenosine 3',5'-diphosphate | - |
? | |
NADP+ + H2O | possible function in regulation of NADP-level | Proteus vulgaris | NMN + adenosine 3',5'-diphosphate | - |
? | |
NADPH + H2O | - |
Proteus vulgaris | ? | - |
? | |
UDP + H2O | - |
Proteus vulgaris | UMP + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Proteus vulgaris |