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Literature summary for 3.6.1.22 extracted from

  • Wagner, R.; Feth, F.; Wagner, K.G.
    The pyridine-nucleotide cycle in tobacco. Enzyme activities for the recycling of NAD (1986), Planta, 167, 226-232.
    View publication on PubMed
Search for more literature for 3.6.1.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NAD+ 100 mM MOPS-NaOH, pH 7.4, 5 mM MgCl2, 10 mM dithioerythritol, 0.1 mM EDTA Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + H2O Nicotiana tabacum enzyme of the pyridine-nucleotide cycle, involved in nicotine production NMN + AMP
-
 ir

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
 callus
-

Source Tissue

Source Tissue Comment Organism Textmining
root callus Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
 partially purified enzyme Nicotiana tabacum

Storage Stability

Storage Stability Organism
4°C, 100 mM MOPS-NaOH, pH 7.4, 5 mM MgCl2, 10 mM dithioerythritol, 0.1 mM EDTA, 5h, no loss of activity Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + H2O
-
 Nicotiana tabacum NMN + AMP
-
 ir
NAD+ + H2O enzyme of the pyridine-nucleotide cycle, involved in nicotine production Nicotiana tabacum NMN + AMP
-
 ir
thymidine 5'-p-nitrophenylphosphate + H2O
-
 Nicotiana tabacum thymidine + 4-nitrophenyl phosphate
-
 ?