BRENDA - Enzyme Database
show all sequences of 3.6.1.22

Purification and properties of NADP pyrophosphatase from Proteus vulgaris

Nakajima, Y.; Fukunaga, N.; Sasaki, S.; Usami, S.; Biochim. Biophys. Acta 293, 242-255 (1973)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2',5'-ADP
44% inhibition at concentration of 0.133 mM
Proteus vulgaris
ADP
21% inhibition at concentration of 0.133 mM
Proteus vulgaris
ATP
22% inhibition at concentration of 0.066 mM
Proteus vulgaris
Ca2+
20% inhibition at concentration of 0.33 mM
Proteus vulgaris
Co2+
40% inhibition of the purified enzyme at concentration of 0.33 mM, activity in crude extract not affected
Proteus vulgaris
CoA
85% inhibition at concentration of 0.133 mM
Proteus vulgaris
CTP
55% inhibition at concentration of 0.066 mM
Proteus vulgaris
DNA
strong inhibition by denaturated DNA, slight inhibition by native DNA
Proteus vulgaris
EDTA
87% inhibition, reactivation by Mn2+ or Co2+
Proteus vulgaris
FAD
35% inhibition at concentration of 0.133 mM
Proteus vulgaris
GTP
53% inhibition at concentration of 0.066 mM
Proteus vulgaris
RNA
strong inhibition
Proteus vulgaris
UDP
32% inhibition at concentration of 0.133 mM
Proteus vulgaris
UTP
36% inhibition at concentration of 0.066 mM
Proteus vulgaris
Zn2+
70% inhibition at concentration of 0.33 mM
Proteus vulgaris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
NADP+
Tris-HCl, pH 7.0
Proteus vulgaris
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
reactivation of EDTA-treated enzyme
Proteus vulgaris
Mg2+
slight activation at concentration of 1.0 mM
Proteus vulgaris
Mn2+
slight activation at concentration of 1.0 mM, reactivation of EDTA-treated enzyme
Proteus vulgaris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NADP+ + H2O
Proteus vulgaris
possible function in regulation of NADP-level
NMN + adenosine 3',5'-diphosphate
-
Proteus vulgaris
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Proteus vulgaris
-
-
-
Purification (Commentary)
Commentary
Organism
-
Proteus vulgaris
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0008
-
crude extract
Proteus vulgaris
0.78
-
purified enzyme with 1 mM MnCl2
Proteus vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
-
209949
Proteus vulgaris
AMP + phosphate
-
209949
Proteus vulgaris
?
AMP + H2O
-
209949
Proteus vulgaris
adenosine + phosphate
-
209949
Proteus vulgaris
?
NAD+ + H2O
-
209949
Proteus vulgaris
NMN + AMP
-
209949
Proteus vulgaris
ir
NADH + H2O
-
209949
Proteus vulgaris
AMP + NMNH
-
-
-
?
NADP+ + H2O
-
209949
Proteus vulgaris
NMN + adenosine 3',5'-diphosphate
-
209949
Proteus vulgaris
?
NADP+ + H2O
possible function in regulation of NADP-level
209949
Proteus vulgaris
NMN + adenosine 3',5'-diphosphate
-
209949
Proteus vulgaris
?
NADPH + H2O
-
209949
Proteus vulgaris
?
-
-
-
?
UDP + H2O
-
209949
Proteus vulgaris
UMP + phosphate
-
209949
Proteus vulgaris
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Proteus vulgaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2',5'-ADP
44% inhibition at concentration of 0.133 mM
Proteus vulgaris
ADP
21% inhibition at concentration of 0.133 mM
Proteus vulgaris
ATP
22% inhibition at concentration of 0.066 mM
Proteus vulgaris
Ca2+
20% inhibition at concentration of 0.33 mM
Proteus vulgaris
Co2+
40% inhibition of the purified enzyme at concentration of 0.33 mM, activity in crude extract not affected
Proteus vulgaris
CoA
85% inhibition at concentration of 0.133 mM
Proteus vulgaris
CTP
55% inhibition at concentration of 0.066 mM
Proteus vulgaris
DNA
strong inhibition by denaturated DNA, slight inhibition by native DNA
Proteus vulgaris
EDTA
87% inhibition, reactivation by Mn2+ or Co2+
Proteus vulgaris
FAD
35% inhibition at concentration of 0.133 mM
Proteus vulgaris
GTP
53% inhibition at concentration of 0.066 mM
Proteus vulgaris
RNA
strong inhibition
Proteus vulgaris
UDP
32% inhibition at concentration of 0.133 mM
Proteus vulgaris
UTP
36% inhibition at concentration of 0.066 mM
Proteus vulgaris
Zn2+
70% inhibition at concentration of 0.33 mM
Proteus vulgaris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
NADP+
Tris-HCl, pH 7.0
Proteus vulgaris
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
reactivation of EDTA-treated enzyme
Proteus vulgaris
Mg2+
slight activation at concentration of 1.0 mM
Proteus vulgaris
Mn2+
slight activation at concentration of 1.0 mM, reactivation of EDTA-treated enzyme
Proteus vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NADP+ + H2O
Proteus vulgaris
possible function in regulation of NADP-level
NMN + adenosine 3',5'-diphosphate
-
Proteus vulgaris
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Proteus vulgaris
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0008
-
crude extract
Proteus vulgaris
0.78
-
purified enzyme with 1 mM MnCl2
Proteus vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + H2O
-
209949
Proteus vulgaris
AMP + phosphate
-
209949
Proteus vulgaris
?
AMP + H2O
-
209949
Proteus vulgaris
adenosine + phosphate
-
209949
Proteus vulgaris
?
NAD+ + H2O
-
209949
Proteus vulgaris
NMN + AMP
-
209949
Proteus vulgaris
ir
NADH + H2O
-
209949
Proteus vulgaris
AMP + NMNH
-
-
-
?
NADP+ + H2O
-
209949
Proteus vulgaris
NMN + adenosine 3',5'-diphosphate
-
209949
Proteus vulgaris
?
NADP+ + H2O
possible function in regulation of NADP-level
209949
Proteus vulgaris
NMN + adenosine 3',5'-diphosphate
-
209949
Proteus vulgaris
?
NADPH + H2O
-
209949
Proteus vulgaris
?
-
-
-
?
UDP + H2O
-
209949
Proteus vulgaris
UMP + phosphate
-
209949
Proteus vulgaris
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Proteus vulgaris
Other publictions for EC 3.6.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733748
Wang
Identification of UshA as a ma ...
Escherichia coli, Escherichia coli DH10B
Enzyme Microb. Technol.
58-59
75-79
2014
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3
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2
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3
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4
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3
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1
1
-
3
3
720498
Wang
Comparative analysis of mycoba ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Mycobacterium tuberculosis variant bovis
Mol. Microbiol.
82
1375-1391
2011
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-
2
-
2
-
10
-
-
3
4
-
-
8
-
-
2
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8
2
1
1
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1
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2
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2
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10
-
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3
4
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2
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8
2
1
1
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1
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2
2
-
-
-
670632
Jambunathan
Analysis of Arabidopsis growth ...
Arabidopsis sp.
Planta
224
1-11
2006
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-
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1
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4
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1
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1
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668217
Magni
Enzymology of NAD+ homeostasis ...
Homo sapiens
Cell. Mol. Life Sci.
61
19-34
2004
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-
-
-
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1
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1
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1
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654332
AbdelRaheim
The NADH diphosphatase encoded ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
388
18-24
2001
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-
1
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3
1
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1
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1
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1
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7
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1
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1
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7
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655747
Schmidt-Brauns
Is a NAD pyrophosphatase activ ...
Haemophilus influenzae
Int. J. Med. Microbiol.
291
219-225
2001
-
-
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1
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4
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2
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1
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2
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1
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656433
Bortell
Nicotinamide adenine dinucleot ...
Rattus norvegicus
J. Immunol.
167
2049-2059
2001
-
1
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1
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1
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1
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1
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1
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1
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209959
Xu
Cloning and characterization o ...
Caenorhabditis elegans, Escherichia coli, Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
273
753-758
2000
-
-
2
-
-
-
4
4
-
6
4
3
-
6
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2
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19
3
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4
3
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2
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4
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4
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6
4
3
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19
3
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4
3
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209958
Hagen
Detection and identification o ...
Rattus norvegicus
Biochim. Biophys. Acta
1340
7-12
1997
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1
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209957
Frick
Cloning, purification, and pro ...
Escherichia coli
J. Biol. Chem.
270
1529-1534
1995
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1
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1
6
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5
2
1
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2
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1
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13
1
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1
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1
1
6
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1
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1
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13
1
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1
1
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171771
Wagner
The pyridine-nucleotide cycle ...
Nicotiana tabacum
Planta
167
226-232
1986
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1
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1
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4
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1
1
1
3
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1
1
3
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171772
Wagner
Regulation in tobacco callus o ...
Nicotiana tabacum
Planta
168
408-413
1986
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1
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1
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1
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209954
Falconer
-
Membrane association of NAD py ...
Salmonella enterica subsp. enterica serovar Typhimurium
Curr. Microbiol.
10
237-242
1984
-
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1
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1
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1
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171773
Foster
Pyridine nucleotide cycle of S ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
145
1002-1009
1981
-
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209952
Berghaeuser
-
A simple preparation of an enz ...
Solanum tuberosum
Biotechnol. Lett.
3
339-344
1981
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1
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1
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209951
Davies
An enzyme degrading reduced ni ...
Proteus vulgaris
Biochem. J.
175
669-674
1978
1
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5
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4
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2
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1
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1
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2
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1
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5
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4
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1
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1
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2
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209950
Jeck
Simple methods of preparing ni ...
Solanum tuberosum
FEBS Lett.
42
161-164
1974
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209949
Nakajima
Purification and properties of ...
Proteus vulgaris
Biochim. Biophys. Acta
293
242-255
1973
-
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15
1
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3
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1
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2
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1
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2
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8
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1
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15
-
1
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3
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1
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1
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2
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8
-
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-
1
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209948
Anderson
Nicotinamide-adenin dinucleoti ...
Aedes aegypti
Biochem. J.
101
392-396
1966
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