BRENDA - Enzyme Database
show all sequences of 3.6.1.18

Flavin nucleotide metabolism in plants: monofunctional enzymes synthesize FAD in plastids

Sandoval, F.J.; Zhang, Y.; Roje, S.; J. Biol. Chem. 283, 30890-30900 (2008)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Pisum sativum
9507
-
mitochondrion
-
Pisum sativum
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
activity is Mg2+-dependent
Pisum sativum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
FAD + H2O
Pisum sativum
-
AMP + FMN
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pisum sativum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Pisum sativum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00003
-
at pH 7.5 in chloroplasts, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin
Pisum sativum
0.0000384
-
at pH 8.5 in chloroplasts, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin
Pisum sativum
0.000343
-
at pH 7.5 in mitochondria, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin
Pisum sativum
0.000482
-
at pH 8.5 in mitochondria, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin
Pisum sativum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
FAD + H2O
-
693166
Pisum sativum
AMP + FMN
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
FAD diphosphatase
-
Pisum sativum
FAD pyrophosphatase
-
Pisum sativum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Pisum sativum
9507
-
mitochondrion
-
Pisum sativum
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
activity is Mg2+-dependent
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
FAD + H2O
Pisum sativum
-
AMP + FMN
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00003
-
at pH 7.5 in chloroplasts, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin
Pisum sativum
0.0000384
-
at pH 8.5 in chloroplasts, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin
Pisum sativum
0.000343
-
at pH 7.5 in mitochondria, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin
Pisum sativum
0.000482
-
at pH 8.5 in mitochondria, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin
Pisum sativum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
FAD + H2O
-
693166
Pisum sativum
AMP + FMN
-
-
-
?
Other publictions for EC 3.6.1.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
757628
Deka
Molecular insights into the e ...
Treponema pallidum, Escherichia coli
MicrobiologyOpen
5
21-38
2016
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-
-
1
3
-
2
-
2
2
-
3
-
12
-
-
-
-
-
1
-
-
6
-
11
2
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-
-
2
-
-
-
-
-
-
-
-
-
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1
3
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-
2
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-
2
2
-
3
-
-
-
-
-
1
-
-
6
-
2
-
-
-
2
-
-
-
-
7
7
-
-
-
734615
Deka
Evidence for posttranslational ...
Treponema pallidum, Treponema pallidum Nichols
mBio
6
e00519
2015
-
-
1
-
9
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
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-
2
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-
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1
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9
-
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1
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2
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-
-
-
-
-
734210
Deka
The TP0796 lipoprotein of Trep ...
Treponema pallidum, Treponema pallidum Nichols
J. Biol. Chem.
288
11106-11121
2013
4
-
1
1
-
-
4
-
-
-
2
-
-
12
-
-
-
-
-
2
-
-
2
1
1
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-
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1
-
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-
-
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4
-
1
-
1
-
-
-
4
-
-
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-
2
-
-
-
-
-
-
2
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
721117
Pallotta
Evidence for the presence of a ...
Saccharomyces cerevisiae
Yeast
28
693-705
2011
-
-
-
-
-
-
3
-
3
-
-
-
-
4
-
-
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-
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-
1
-
2
-
-
-
-
-
-
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-
1
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-
-
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-
1
3
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-
3
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
693166
Sandoval
Flavin nucleotide metabolism i ...
Pisum sativum
J. Biol. Chem.
283
30890-30900
2008
-
-
-
-
-
-
-
-
2
1
-
1
-
9
-
-
-
-
-
1
4
-
1
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2
-
-
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2
1
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1
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1
4
-
1
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-
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-
-
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-
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-
95064
Lee
Purification and characterizat ...
Homo sapiens
Methods Enzymol.
280
424-436
1997
-
-
-
-
-
-
1
-
1
2
3
1
-
1
-
1
1
-
-
1
-
-
1
-
-
-
-
1
-
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-
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-
1
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-
1
2
3
1
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1
1
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1
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-
1
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-
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1
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-
-
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-
209924
Barile
Flavin adenine dinucleotide an ...
Rattus norvegicus
Eur. J. Biochem.
249
777-785
1997
-
-
-
-
-
-
6
1
3
2
-
1
-
4
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
1
-
-
-
-
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-
-
-
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-
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6
-
1
3
2
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
209923
Kim
Purification and characterizat ...
Rattus norvegicus
J. Biochem.
114
126-131
1993
-
-
-
-
-
-
4
1
1
2
2
1
-
1
-
-
1
-
-
1
2
-
3
1
-
-
-
-
-
1
1
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-
-
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-
-
-
-
-
-
-
-
-
4
-
1
1
2
2
1
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1
-
1
2
-
3
1
-
-
-
-
1
1
-
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-
-
-
-
-
-
209921
Byrd
Rat intestinal nucleotide-suga ...
Rattus norvegicus
J. Biol. Chem.
260
7474-7480
1985
-
-
-
-
-
-
4
-
1
-
2
1
-
2
-
-
1
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-
1
1
-
3
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2
-
1
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-
-
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-
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4
-
-
1
-
2
1
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-
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1
-
1
1
-
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
209922
Okumura
-
Purification and properties of ...
Oryctolagus cuniculus
Nagoya Igaku
105
117-123
1983
-
-
-
-
-
-
1
1
-
1
1
1
-
1
-
-
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-
-
1
-
-
1
-
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1
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-
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1
-
-
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-
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-
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-
-
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1
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1
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1
1
1
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1
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-
1
-
1
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-
-
1
-
-
-
-
-
-
-
-
-
209920
Akiyama
FMN phosphatase and FAD pyroph ...
Rattus norvegicus
J. Nutr.
112
263-268
1982
-
-
-
-
-
-
1
-
3
-
-
1
-
4
-
-
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-
3
1
-
1
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-
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-
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1
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1
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3
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1
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3
1
-
1
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
209919
Ravindranath
Nucleotidases in plants: V. Pu ...
Vigna radiata var. radiata
Indian J. Biochem. Biophys.
8
219-226
1971
1
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
1
-
-
-
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-
6
-
-
1
-
-
-
1
-
-
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1
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-
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1
-
1
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1
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1
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-
-
6
-
1
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-
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1
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-
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-
-
-
-
-
-
209918
Mistuda
On the enzymatic hydrolysis of ...
Spinacia oleracea
J. Vitaminol. (Kyoto)
16
31-38
1970
-
-
-
-
-
-
11
1
-
5
-
1
-
1
-
-
-
-
-
-
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6
-
-
1
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1
1
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-
-
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-
-
-
-
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-
-
11
-
1
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5
-
1
-
-
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-
-
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-
6
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
209917
Ravindranath
Nucleotidases in plants. III. ...
Vigna radiata var. radiata
Arch. Biochem. Biophys.
133
54-59
1969
2
-
-
-
-
-
7
1
-
-
-
1
-
1
-
-
1
-
1
-
-
1
1
-
-
1
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-
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1
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-
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-
-
2
-
-
-
-
-
-
-
7
-
1
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1
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1
1
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1
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
209915
Ragab
Hydrolysis of flavin-adenine d ...
Rattus norvegicus
Arch. Biochem. Biophys.
123
179-185
1968
-
-
-
-
-
-
7
1
1
1
1
1
-
1
-
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1
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1
1
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1
-
-
-
-
-
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1
2
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7
-
1
1
1
1
1
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1
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1
1
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1
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-
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1
2
-
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-
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-
-
-
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209916
Ravindranath
-
Studies on nucleotidases in pl ...
Vigna radiata var. radiata
Indian J. Biochem.
5
137-142
1968
-
-
-
-
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7
3
-
6
-
1
-
1
-
-
-
-
-
-
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1
3
-
-
1
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-
-
1
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7
-
3
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6
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1
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1
3
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1
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1
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