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Literature summary for 3.6.1.17 extracted from
- Molecular characterization and mutational analysis of recombinant diadenosine 5',5''-P1,P4-tetraphosphate hydrolase from Plasmodium falciparum (2012), Biol. Pharm. Bull., 35, 1191-1196.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Plasmodium falciparum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| P133A | 5fold increase in Km value | Plasmodium falciparum |
| P133F | 3fold decrease in kcat value | Plasmodium falciparum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
5',5''-diadenosine tetraphosphate | wild-type, pH 7.5, 22°C | Plasmodium falciparum |  |
| 0.0008 | - |
5',5''-diadenosine tetraphosphate | mutant P133F, pH 7.5, 22°C | Plasmodium falciparum | |
| 0.003 | - |
5',5''-diadenosine tetraphosphate | mutant P133A, pH 7.5, 22°C | Plasmodium falciparum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, 42% of the activity with Mg2+ | Plasmodium falciparum | |
| Mg2+ | best activator, maximum activity at 5 mM | Plasmodium falciparum | |
| Mn2+ | 5 mM, 11% of the activity with Mg2+ | Plasmodium falciparum | |
| additional information | no activation by Ca2+ | Plasmodium falciparum | |
| Zn2+ | 5 mM, 2% of the activity with Mg2+ | Plasmodium falciparum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
x * 17000, SDS-PAGE | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | C0H4F3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5',5''-diadenosine pentaphosphate + H2O | - |
Plasmodium falciparum | ATP + ADP | 42% of the activity with 5',5''-diadenosine tetraphosphate | ? | |
| 5',5''-diadenosine tetraphosphate + H2O | - |
Plasmodium falciparum | ATP + AMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 17000, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFE1035c | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Plasmodium falciparum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, 39% of initial activity | Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
5',5''-diadenosine tetraphosphate | mutant P133F, pH 7.5, 22°C | Plasmodium falciparum | |
| 0.042 | - |
5',5''-diadenosine tetraphosphate | wild-type, pH 7.5, 22°C | Plasmodium falciparum | |
| 0.08 | - |
5',5''-diadenosine tetraphosphate | mutant P133A, pH 7.5, 22°C | Plasmodium falciparum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Plasmodium falciparum |
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