Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)T cells | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
diadenosine 5',5''''-P1,P4-tetraphosphate | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
0.0986 | - |
8-oxo-dGTP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
0.1143 | - |
dATP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
0.182 | - |
dGTP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
0.3846 | - |
2-oxo-dATP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | Q9U2M7 | - |
- |
Caenorhabditis elegans Bristol N2 | Q9U2M7 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap FF column chromatography and Superdex 75 gel filtration | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-dATP + H2O | less efficient hydrolysis compared to 8-oxo-dGTP | Caenorhabditis elegans | ? | - |
? | |
2-oxo-dATP + H2O | less efficient hydrolysis compared to 8-oxo-dGTP | Caenorhabditis elegans Bristol N2 | ? | - |
? | |
8-oxo-dGTP + H2O | the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency | Caenorhabditis elegans | 8-oxo-dGMP + diphosphate | - |
? | |
8-oxo-dGTP + H2O | the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency | Caenorhabditis elegans Bristol N2 | 8-oxo-dGMP + diphosphate | - |
? | |
dATP + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
dATP + H2O | - |
Caenorhabditis elegans Bristol N2 | ? | - |
? | |
dGTP + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
dGTP + H2O | - |
Caenorhabditis elegans Bristol N2 | ? | - |
? | |
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O | - |
Caenorhabditis elegans | AMP + ATP | - |
? | |
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O | - |
Caenorhabditis elegans Bristol N2 | AMP + ATP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NDX-4 | - |
Caenorhabditis elegans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
dATP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
0.51 | - |
2-oxo-dATP | in 5 0mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
0.53 | - |
dGTP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
0.58 | - |
8-oxo-dGTP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
27 | - |
diadenosine 5',5''''-P1,P4-tetraphosphate | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of NDX-4 leads to upregulation of key stress responsive genes that likely compensate for the in vivo role of NDX-4 in protection against deleterious consequences of oxidative stress. NDX-4 deficiency protects nematodes from acute heat shock | Caenorhabditis elegans |
physiological function | the enzyme contributes to genomic stability in vivo in Caenorhabditis elegans | Caenorhabditis elegans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
2-oxo-dATP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
2.9 | - |
dGTP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
3.3 | - |
dATP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
5.9 | - |
8-oxo-dGTP | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans | |
3900 | - |
diadenosine 5',5''''-P1,P4-tetraphosphate | in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C | Caenorhabditis elegans |