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Literature summary for 3.6.1.17 extracted from

  • Winward, L.; Whitfield, W.G.; Woodman, T.J.; McLennan, A.G.; Safrany, S.T.
    Characterisation of a bis(5-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster (2007), Int. J. Biochem. Cell Biol., 39, 943-954.
    View publication on PubMed
Search for more literature for 3.6.1.17

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison, expression of His-tagged Apf in Spodoptera frugiperda Sf21 cells, expression of EGFP-tagged enzyme in Drosophila melanbogaster Mel-2 cells Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
fluoride fluoride potently inhibits diadenosine tetraphosphate hydrolysis in the presence of Mg2+, whereas it is ineffective in the presence of Zn2+, because inhibition involves a specific, MgF3--containing transition state analogue complex, overview Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics strongly depend on pH and cation, overview Drosophila melanogaster
0.009
-
 5',5'-diadenosine tetraphosphate pH 6.5, 1 mm Zn2+ Drosophila melanogaster
0.012
-
 5',5'-diadenosine tetraphosphate pH 7.5, 20 mM Mg2+ Drosophila melanogaster
0.015
-
 5',5'-diadenosine hexaphosphate pH 7.5, 20 mM Mg2+ Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus association with euchromatin and facultative heterochromatin Drosophila melanogaster 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the enzyme is dependent on divalent cations Drosophila melanogaster
Mg2+ the enzyme is dependent on divalent cations, preferred cation, best at 3-10 mM Drosophila melanogaster
Mn2+ the enzyme is dependent on divalent cations Drosophila melanogaster
additional information no activation by Cu2+, Fe2+, Ni2+ or Ca2+ Drosophila melanogaster
Zn2+ the enzyme is dependent on divalent cations, high activity Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5',5'-diadenosine tetraphosphate + H2O Drosophila melanogaster
-
 ATP + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q4V6M1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Apf from Spodoptera frugiperda Sf21 cells by nickel affinity chromatography with removal of the His-tag Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
additional information patterns of Apf expression in Drosophila melanogaster tissues, semi-quantitative RT-PCR, highest expression levels in embryos and adult females, overview Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5',5'-diadenosine hexaphosphate + H2O diadenosine hexaphosphate is efficiently hydrolysed to ATP only at pH 7.5 with 20 mM Mg2+ Drosophila melanogaster 2 ATP
-
 ?
5',5'-diadenosine pentaphosphate + H2O
-
 Drosophila melanogaster ATP + ADP
-
 ?
5',5'-diadenosine tetraphosphate + H2O
-
 Drosophila melanogaster ATP + AMP
-
 ?
5',5'-diadenosine tetraphosphate + H2O best substrate Drosophila melanogaster ATP + AMP
-
 ?
adenosine-5'-pentaphospho-5'-guanosine + H2O
-
 Drosophila melanogaster ?
-
 ?
adenosine-5'-tetraphospho-5'-guanosine + H2O
-
 Drosophila melanogaster ?
-
 ?
additional information Apf always produces an NTP product, with substrate preference depending on pH and divalent ion Drosophila melanogaster ?
-
 ?

Subunits

Subunits Comment Organism
More the enzyme contains a Nudix motif Drosophila melanogaster

Synonyms

Synonyms Comment Organism
Ap4A hydrolase
-
 Drosophila melanogaster
Apf
-
 Drosophila melanogaster
More Apf is a typical member of the bis (5'-nucleosyl)-tetraphosphatase subfamily of Nudix hydrolases Drosophila melanogaster
Nudix hydrolase
-
 Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 40 broad optimum Drosophila melanogaster

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 65% of maximal activity at 52°C Drosophila melanogaster

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 20 min, 55% remaining activity Drosophila melanogaster
95
-
 20 min, 1 mM Mn2+, a little amount of activity is remaining Drosophila melanogaster

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 5',5'-diadenosine hexaphosphate pH 7.5, 20 mM Mg2+ Drosophila melanogaster
13
-
 5',5'-diadenosine tetraphosphate pH 7.5, 20 mM Mg2+ Drosophila melanogaster
43
-
 5',5'-diadenosine tetraphosphate pH 6.5, 1 mM Zn2+ Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 activity depends on the substrate and the divalent cation Drosophila melanogaster

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.02
-
 in presence of Mg2+ Drosophila melanogaster fluoride