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Literature summary for 3.6.1.17 extracted from

  • Abdelghany, H.M.; Bailey, S.; Blackburn, G.M.; Rafferty, J.B.; McLennan, A.G.
    Analysis of the catalytic and binding residues of the diadenosine tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans by site-directed mutagenesis (2003), J. Biol. Chem., 278, 4435-4439.
    View publication on PubMed
Search for more literature for 3.6.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, mutant enzymes Y27A, Y27D, H31A, H31V, W32G, K36M, H38G, H38K, E52Q, E56Q, Y76A, Y76/Y121A, K79M, K81M, K83M, E103Q, Y121A Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
E103Q turnover-number for hydrolysis of Ap4A is 3.2% of that of the wild-type enzyme, KM-value is 66% of the wild-type value Caenorhabditis elegans
E52Q turnover-number for hydrolysis of Ap4A is 0.02% of that of the wild-type enzyme, KM-value is 60% of the wild-type value Caenorhabditis elegans
E56Q turnover-number for hydrolysis of Ap4A is 0.001% of that of the wild-type enzyme, KM-value is 90% of the wild-type value Caenorhabditis elegans
H31A turnover-number for hydrolysis of Ap4A is 52% of that of the wild-type enzyme, KM-value is 8.3fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
H31V turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 12.5fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
H38G turnover-number for hydrolysis of Ap4A is 135% of that of the wild-type enzyme, KM-value is 66% of the wild-type value Caenorhabditis elegans
H38K turnover-number for hydrolysis of Ap4A is 135% of that of the wild-type enzyme, KM-value is 47% of the wild-type value Caenorhabditis elegans
K36M turnover-number for hydrolysis of Ap4A is 9% of that of the wild-type enzyme, KM-value is 1.5fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
K79M turnover-number for hydrolysis of Ap4A is 0.7% of that of the wild-type enzyme, KM-value is 1.7fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
K81M turnover-number for hydrolysis of Ap4A is 130% of that of the wild-type enzyme, KM-value is 2.3fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
K83M turnover-number for hydrolysis of Ap4A is 2.6% of that of the wild-type enzyme, KM-value is 15.9fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
Y121A turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 8.5fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
Y27A turnover-number for hydrolysis of Ap4A is 113% of that of the wild-type enzyme, KM-value is 1.25fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
Y27D turnover-number for hydrolysis of Ap4A is 126% of that of the wild-type enzyme, KM-value is 1.8fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
Y76A turnover-number for hydrolysis of Ap4A is 8.7% of that of the wild-type enzyme, KM-value is 6.7fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
Y76A/Y121A turnover-number for hydrolysis of Ap4A is 4.8% of that of the wild-type enzyme, KM-value is 3.75fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
 Ap4A mutant enzyme H38K Caenorhabditis elegans
0.0053
-
 Ap4A mutant enzyme E52Q Caenorhabditis elegans
0.0058
-
 Ap4A mutant enzyme E103Q Caenorhabditis elegans
0.0058
-
 Ap4A mutant enzyme H38G Caenorhabditis elegans
0.0079
-
 Ap4A mutant enzyme E56Q Caenorhabditis elegans
0.0088
-
 Ap4A wild-type enzyme Caenorhabditis elegans
0.011
-
 Ap4A mutant enzyme Y27A Caenorhabditis elegans
0.013
-
 Ap4A mutant enzyme K36M Caenorhabditis elegans
0.015
-
 Ap4A mutant enzyme K79M Caenorhabditis elegans
0.016
-
 Ap4A mutant enzyme Y27D Caenorhabditis elegans
0.02
-
 Ap4A mutant enzyme K81M Caenorhabditis elegans
0.033
-
 Ap4A mutant enzyme Y76A/Y121A Caenorhabditis elegans
0.059
-
 Ap4A mutant enzyme Y76A Caenorhabditis elegans
0.073
-
 Ap4A mutant enzyme H31A Caenorhabditis elegans
0.075
-
 Ap4A mutant enzyme Y121A Caenorhabditis elegans
0.11
-
 Ap4A mutant enzyme H31V Caenorhabditis elegans
0.14
-
 Ap4A mutant enzyme K83M Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ap4A + H2O
-
 Caenorhabditis elegans ?
-
 ?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
 Caenorhabditis elegans ATP + AMP
-
 ?

Synonyms

Synonyms Comment Organism
diadenosine tetraphosphate pyrophosphohydrolase
-
 Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00024
-
 Ap4A mutant enzyme E56Q Caenorhabditis elegans
0.0052
-
 Ap4A mutant enzyme E52Q Caenorhabditis elegans
0.16
-
 Ap4A mutant enzyme K79M Caenorhabditis elegans
0.6
-
 Ap4A mutant enzyme K83M Caenorhabditis elegans
0.75
-
 Ap4A mutant enzyme E103Q Caenorhabditis elegans
1.1
-
 Ap4A mutant enzyme Y76A/Y121A Caenorhabditis elegans
2
-
 Ap4A mutant enzyme Y76A Caenorhabditis elegans
2.1
-
 Ap4A mutant enzyme K36M Caenorhabditis elegans
12
-
 Ap4A mutant enzyme H31A Caenorhabditis elegans
14
-
 Ap4A mutant enzyme H31V Caenorhabditis elegans
14
-
 Ap4A mutant enzyme Y121A Caenorhabditis elegans
23
-
 Ap4A wild-type enzyme Caenorhabditis elegans
26
-
 Ap4A mutant enzyme Y27A Caenorhabditis elegans
29
-
 Ap4A mutant enzyme Y27D Caenorhabditis elegans
30
-
 Ap4A mutant enzyme K81M Caenorhabditis elegans
31
-
 Ap4A mutant enzyme H38G Caenorhabditis elegans
31
-
 Ap4A mutant enzyme H38K Caenorhabditis elegans