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Literature summary for 3.6.1.17 extracted from

  • Abdelghany, H.M.; Gasmi, L.; Cartwright, J.L.; Bailey, S.; Rafferty, J.B.; McLennan, A.G.
    Cloning, characterisation and crystallization of a diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans (2001), Biochim. Biophys. Acta, 1550, 27-36.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Caenorhabditis elegans

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour diffusion, crystals diffract to a minimum d-spacing of 2 A and belong to either space group C222 or C222(1) Caenorhabditis elegans

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-tetraphosphate competitive Caenorhabditis elegans
F- non-competitive Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
Ap4A pH 7.8, 25°C Caenorhabditis elegans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required, 20% of maximal activity in presence of 1 mM Co2+ Caenorhabditis elegans
Mg2+ divalent cation required, maximal activity in presence of 3-5 mM Mg2+ Caenorhabditis elegans
Mn2+ divalent cation required, 20% of maximal activity in presence of 0.1 mM Mn2+ Caenorhabditis elegans
Zn2+ divalent cation required, 20% of maximal activity in presence of 0.05 mM Mn2+ Caenorhabditis elegans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
gel filtration Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ap4A + H2O
-
Caenorhabditis elegans ?
-
?
Ap6A + H2O 7% of the activity with Ap4A Caenorhabditis elegans ATP + ATP
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
Caenorhabditis elegans ATP + AMP
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O 19% of the activity with Ap4A Caenorhabditis elegans ATP + ADP
-
?
additional information no activity with Ap3A Caenorhabditis elegans ?
-
?
p4A + H2O 14% of the activity with Ap4A Caenorhabditis elegans ATP + phosphate
-
?
p5A + H2O 29% of the activity with Ap4A Caenorhabditis elegans ATP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
Ap4A hydrolase
-
Caenorhabditis elegans
diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase
-
Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27
-
Ap4A pH 7.8, 25°C Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
hydrolysis pf Ap4A Caenorhabditis elegans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 50% of maximal activity at pH 6.5 and at pH 8.0, hydrolysis of Ap4A Caenorhabditis elegans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001
-
adenosine 5'-tetraphosphate pH 7.8, 25°C Caenorhabditis elegans
0.025
-
F- pH 7.8, 25°C Caenorhabditis elegans