Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Caenorhabditis elegans |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour diffusion, crystals diffract to a minimum d-spacing of 2 A and belong to either space group C222 or C222(1) | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-tetraphosphate | competitive | Caenorhabditis elegans | |
F- | non-competitive | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
Ap4A | pH 7.8, 25°C | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required, 20% of maximal activity in presence of 1 mM Co2+ | Caenorhabditis elegans | |
Mg2+ | divalent cation required, maximal activity in presence of 3-5 mM Mg2+ | Caenorhabditis elegans | |
Mn2+ | divalent cation required, 20% of maximal activity in presence of 0.1 mM Mn2+ | Caenorhabditis elegans | |
Zn2+ | divalent cation required, 20% of maximal activity in presence of 0.05 mM Mn2+ | Caenorhabditis elegans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
gel filtration | Caenorhabditis elegans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ap4A + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
Ap6A + H2O | 7% of the activity with Ap4A | Caenorhabditis elegans | ATP + ATP | - |
? | |
diadenosine 5',5''-P1,P4-tetraphosphate + H2O | - |
Caenorhabditis elegans | ATP + AMP | - |
? | |
diadenosine 5',5''-P1,P5-pentaphosphate + H2O | 19% of the activity with Ap4A | Caenorhabditis elegans | ATP + ADP | - |
? | |
additional information | no activity with Ap3A | Caenorhabditis elegans | ? | - |
? | |
p4A + H2O | 14% of the activity with Ap4A | Caenorhabditis elegans | ATP + phosphate | - |
? | |
p5A + H2O | 29% of the activity with Ap4A | Caenorhabditis elegans | ATP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ap4A hydrolase | - |
Caenorhabditis elegans |
diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase | - |
Caenorhabditis elegans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27 | - |
Ap4A | pH 7.8, 25°C | Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
hydrolysis pf Ap4A | Caenorhabditis elegans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | 50% of maximal activity at pH 6.5 and at pH 8.0, hydrolysis of Ap4A | Caenorhabditis elegans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001 | - |
adenosine 5'-tetraphosphate | pH 7.8, 25°C | Caenorhabditis elegans | |
0.025 | - |
F- | pH 7.8, 25°C | Caenorhabditis elegans |