Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-tetraphosphate | competitive; Ki in nanomolar range | Photinus pyralis | |
fluoride | Ki: 0.05; non-competitive | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | - |
Photinus pyralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14500 | - |
gel filtration | Photinus pyralis |
16000 | - |
SDS-PAGE | Photinus pyralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | Photinus pyralis | possible role as reporter for luciferase gene | ATP + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
firefly | - |
Purification (Comment) | Organism |
---|---|
- |
Photinus pyralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
crude extract | Photinus pyralis |
13.5 | - |
purified enzyme | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | - |
Photinus pyralis | ATP + AMP | - |
? | |
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | possible role as reporter for luciferase gene | Photinus pyralis | ATP + AMP | - |
? | |
P1,P5-bis(5'-adenosyl) pentaphosphate + H2O | - |
Photinus pyralis | ADP + ATP | - |
? | |
P1,P6-bis(5'-adenosyl) hexaphosphate + H2O | - |
Photinus pyralis | 2 ATP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 16000, SDS-PAGE, gel filtration | Photinus pyralis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | - |
Photinus pyralis |