Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. ATP-dependent assays | Toxoplasma gondii |
additional information | generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. Toxoplasma gondii strains null for both NTPase I and NTPase II can be used to avoid interference artifacts in ATP-dependent assays | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q27893 | - |
- |
Toxoplasma gondii | Q27895 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | maintained in human foreskin fibroblast (HFF) cell monolayers in regular growth media | Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzymatic effect of NTPases is independent of the species or cell type background of the reporter assay, as reflected by very similar results in both murine RAW264.7 macrophages and human HeLa cells | Toxoplasma gondii | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
NTP1 | - |
Toxoplasma gondii |
NTP3 | - |
Toxoplasma gondii |
NTPase 1 | - |
Toxoplasma gondii |
NTPase 2 | - |
Toxoplasma gondii |
NTPase-I | UniProt | Toxoplasma gondii |
NTPase-II | UniProt | Toxoplasma gondii |
nucleoside triphosphate hydrolases I | UniProt | Toxoplasma gondii |
nucleoside triphosphate hydrolases II | UniProt | Toxoplasma gondii |
nucleoside-triphosphatase 1 | - |
Toxoplasma gondii |
nucleoside-triphosphatase 2 | - |
Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
malfunction | knockouts of isozymes NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. In contrast to the NTPase I mutant, deletion of NTPase II causes only a mild change in inhibition of the FLUC activity in both HeLa cells and RAW-264.7 macrophages | Toxoplasma gondii |
malfunction | knockouts of isozymes NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. The NTPase I knockout in the type I strain mirrors the less inhibitory phenotype of type II (ME49) and III (CTG) strains, which naturally lack NTPase I | Toxoplasma gondii |
physiological function | strong negative influence of NTPase I on the FLUC activity in both HeLa cells and RAW-264.7 macrophages | Toxoplasma gondii |