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Literature summary for 3.6.1.15 extracted from

  • Olias, P.; Sibley, L.D.
    Functional analysis of the role of Toxoplasma gondii nucleoside triphosphate hydrolases I and II in acute mouse virulence and immune suppression (2016), Infect. Immun., 84, 1994-2001 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.15

Protein Variants

Protein Variants Comment Organism
additional information generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. ATP-dependent assays Toxoplasma gondii
additional information generation of NTPase single- and double-disruptant mutants using CRISPR/Cas9 technology, the disruptant mutants lack either NTPase I (DELTAntpase I, RHDELTAhxgprt/ntpaseI::DHFR) or NTPase II (DELTAntpase II, RHDELTAhxgprt/ntpaseII::DHFR) or both. Knockouts of NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. Toxoplasma gondii strains null for both NTPase I and NTPase II can be used to avoid interference artifacts in ATP-dependent assays Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii Q27893
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Toxoplasma gondii Q27895
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Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite maintained in human foreskin fibroblast (HFF) cell monolayers in regular growth media Toxoplasma gondii
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzymatic effect of NTPases is independent of the species or cell type background of the reporter assay, as reflected by very similar results in both murine RAW264.7 macrophages and human HeLa cells Toxoplasma gondii ?
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Synonyms

Synonyms Comment Organism
NTP1
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 Toxoplasma gondii
NTP3
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 Toxoplasma gondii
NTPase 1
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 Toxoplasma gondii
NTPase 2
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 Toxoplasma gondii
NTPase-I UniProt Toxoplasma gondii
NTPase-II UniProt Toxoplasma gondii
nucleoside triphosphate hydrolases I UniProt Toxoplasma gondii
nucleoside triphosphate hydrolases II UniProt Toxoplasma gondii
nucleoside-triphosphatase 1
-
 Toxoplasma gondii
nucleoside-triphosphatase 2
-
 Toxoplasma gondii

General Information

General Information Comment Organism
malfunction knockouts of isozymes NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. In contrast to the NTPase I mutant, deletion of NTPase II causes only a mild change in inhibition of the FLUC activity in both HeLa cells and RAW-264.7 macrophages Toxoplasma gondii
malfunction knockouts of isozymes NTPase II and I are not essential for growth or virulence in CD1 mice and do not affect STAT1 transcription. The NTPase I knockout in the type I strain mirrors the less inhibitory phenotype of type II (ME49) and III (CTG) strains, which naturally lack NTPase I Toxoplasma gondii
physiological function strong negative influence of NTPase I on the FLUC activity in both HeLa cells and RAW-264.7 macrophages Toxoplasma gondii