Any feedback?
Literature summary for 3.6.1.15 extracted from
- Characterization of the NTPase, RNA-binding, and RNA helicase activities of the DEAH-box splicing factor Prp22 (2005), Biochemistry, 44, 9795-9803.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Poly(A) | stimulates the ATP hydrolysis dependent on the chain length, highest activation by poly(A)40 comapred to shorter molecules | Saccharomyces cerevisiae |  |
| RNA | stimulates the ATP hydrolysis dependent on the chain length | Saccharomyces cerevisiae | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged DEAH-box Prp22 protein in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
| 0.000028 | - |
ATP | pH 7.5, 23°C, in presence of poly(A)40 | Saccharomyces cerevisiae | |
| 0.132 | - |
GTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.138 | - |
ATP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.144 | - |
dGTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.147 | - |
dATP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.147 | - |
dTTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.179 | - |
dCTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.239 | - |
CTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.297 | - |
UTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | the DEAH-box splicing factor Prp22 is important in the second transesterification step of pre-mRNA splicing and is essential for release of mature mRNA from the splicosome | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged DEAH-box Prp22 protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
| CTP + H2O | - |
Saccharomyces cerevisiae | CDP + phosphate | - |
? | |
| dATP + H2O | - |
Saccharomyces cerevisiae | dADP + phosphate | - |
? | |
| dCTP + H2O | - |
Saccharomyces cerevisiae | dCDP + phosphate | - |
? | |
| dGTP + H2O | - |
Saccharomyces cerevisiae | dGDP + phosphate | - |
? | |
| dTTP + H2O | - |
Saccharomyces cerevisiae | dTDP + phosphate | - |
? | |
| GTP + H2O | - |
Saccharomyces cerevisiae | GDP + phosphate | - |
? | |
| additional information | the DEAH-box splicing factor Prp22 is important in the second transesterification step of pre-mRNA splicing and is essential for release of mature mRNA from the splicosome | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | DEAH-box splicing factor Prp22 shows NTPase, RNA-binding, and RNA helicase activities, ATP binding leads to dissociation of the protein from RNA, RNA binding activates the NTPase activity | Saccharomyces cerevisiae | ? | - |
? | |
| ssDNA + H2O | removal of gamma-phosphate from nucleotides | Saccharomyces cerevisiae | ? | - |
? | |
| ssRNA + H2O | removal of gamma-phosphate from nucleotides | Saccharomyces cerevisiae | ? | - |
? | |
| UTP + H2O | - |
Saccharomyces cerevisiae | UDP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DEAH-box Prp22 protein | - |
Saccharomyces cerevisiae |
| DEAH-box splicing factor Prp22 | - |
Saccharomyces cerevisiae |
| NTPase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | 1.97 | GTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.057 | 0.65 | dATP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.5 | - |
dCTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.93 | - |
ATP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.97 | - |
CTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 0.97 | - |
UTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 9.03 | - |
GTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 10.2 | - |
dGTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 10.25 | - |
ATP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 10.9 | - |
dTTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 11.18 | - |
dATP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 12.57 | - |
dCTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 16.67 | - |
CTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
| 17.28 | - |
UTP | pH 7.5, 23°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ssay at | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
