Application | Comment | Organism |
---|---|---|
medicine | considered attractive target for anti-hepatitis C virus chemoterapy | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',3'-didehydro-thymidine triphosphate | competitive to ATP | Hepacivirus C | |
2',3'-dideoxy-5-fluorocytidine triphosphate | competitive to ATP | Hepacivirus C | |
3'-azido-2',3'-dideoxythymidine triphosphate | competitive to ATP | Hepacivirus C | |
ATP | - |
Hepacivirus C | |
dATP | competitive to ATP | Hepacivirus C | |
dCTP | competitive to ATP | Hepacivirus C | |
ddCTP | competitive to ATP | Hepacivirus C | |
ddTTP | competitive to ATP | Hepacivirus C | |
dGTP | competitive to ATP | Hepacivirus C | |
dTTP | competitive to ATP | Hepacivirus C | |
L-dATP | competitive to ATP | Hepacivirus C | |
L-dCTP | competitive to ATP | Hepacivirus C | |
L-ddCTP | competitive to ATP | Hepacivirus C | |
L-dTTP | competitive to ATP | Hepacivirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
ATP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
0.54 | - |
ATP | pH 7.5, 25°C, NTPase activity | Hepacivirus C | |
0.8 | - |
2',3'-didehydro-thymidine triphosphate | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
0.8 | - |
L-dATP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
0.9 | - |
ddTTP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
2.3 | - |
3'-azido-2',3'-dideoxythymidine triphosphate | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
2.4 | - |
dTTP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
3.8 | - |
ddCTP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
4 | - |
dCTP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
6.9 | - |
dATP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
9.9 | - |
L-dCTP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C | |
28 | - |
L-dTTP | pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity | Hepacivirus C |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Hepacivirus C | multifunctional protein, possessing protease, NTPase and helicase activities. These activities are essential for the virus life cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-didehydro-thymidine triphosphate + H2O | - |
Hepacivirus C | 2',3'-didehydro-thymidine diphosphate + phosphate | - |
? | |
3'-azido-2',3'-dideoxythymidine triphosphate + H2O | - |
Hepacivirus C | 3'-azido-2',3'-dideoxythymidine diphosphate + phosphate | - |
? | |
ATP + H2O | - |
Hepacivirus C | ADP + phosphate | - |
? | |
dATP + H2O | - |
Hepacivirus C | dADP + phosphate | - |
? | |
dCTP + H2O | - |
Hepacivirus C | dCDP + phosphate | - |
? | |
ddCTP + H2O | - |
Hepacivirus C | ddCDP + phosphate | - |
? | |
ddTTP + H2O | - |
Hepacivirus C | ddTDP + phosphate | - |
? | |
dTTP + H2O | - |
Hepacivirus C | TDP + phosphate | - |
? | |
L-dATP + H2O | - |
Hepacivirus C | L-dADP + phosphate | - |
? | |
L-dCTP + H2O | - |
Hepacivirus C | CDP + phosphate | - |
? | |
L-dTTP + H2O | - |
Hepacivirus C | L-dTDP + phosphate | - |
? | |
additional information | multifunctional protein, possessing protease, NTPase and helicase activities. These activities are essential for the virus life cycle | Hepacivirus C | ? | - |
? | |
additional information | multifunctional protein, possessing protease, NTPase and helicase activities. Different stereoselectivity in nucleoside triphosphate utilisation suggests that NTPase and helicase activities are coupled by a nucleotide-dependent rate limiting step | Hepacivirus C | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
ATP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
0.6 | - |
dATP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
0.6 | - |
2',3'-didehydro-thymidine triphosphate | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
1.2 | - |
ddTTP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
1.2 | - |
L-dATP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
1.3 | - |
L-dTTP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
1.5 | - |
L-dCTP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
2.1 | - |
3'-azido-2',3'-dideoxythymidine triphosphate | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
2.3 | - |
L-ddCTP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
2.5 | - |
2',3'-dideoxy-5-fluorocytidine triphosphate | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
2.8 | - |
dTTP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
3 | - |
dCTP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
3.5 | - |
dGTP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C | |
3.8 | - |
ddCTP | pH 7.5, 25°C, inhibition of ATPase activity | Hepacivirus C |