Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
in complex with alpha,beta-methyleneadenosine diphosphoribose, sitting drop vapor diffusion method, using 18% (w/v) PEG 4000, 0.1 M sodium acetate buffer pH 5.3, 20% (w/v) glycerol, 0.2 M ammonium sulfate, at 20°C | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermus thermophilus | |
Zn2+ | required | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q84CU3 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q84CU3 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Toyopearl Phenyl-650M column chromatography, and Q Sepharose column chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-ribose + H2O | highly specific substrate | Thermus thermophilus | AMP + D-ribose 5-phosphate | - |
? | |
ADP-ribose + H2O | highly specific substrate | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + D-ribose 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribose pyrophosphatase-I | - |
Thermus thermophilus |
ADPRase-I | - |
Thermus thermophilus |
Ndx4 | - |
Thermus thermophilus |