Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
14-alpha-triptolide | - |
Homo sapiens | |
5-hydroxytriptonide | - |
Homo sapiens | |
triptolide | noncompetitive inhibition | Homo sapiens | |
triptonide | - |
Homo sapiens | |
triptriolide | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.239 | - |
5-iodo-dCTP | in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
2 * 18000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography and Mono Q column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-iodo-dCTP + H2O | good substrate | Homo sapiens | 5-iodo-dCMP + diphosphate | - |
? | |
additional information | gemcitabine triphosphate is no substrate | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 18000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dCTP pyrophosphatase 1 | - |
Homo sapiens |
DCTPP1 | formerly known as XTP3TPA | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000864 | - |
5-iodo-dCTP | in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
triptriolide | in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C | Homo sapiens | |
0.135 | - |
triptonide | in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C | Homo sapiens | |
0.168 | - |
triptolide | in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C | Homo sapiens | |
0.356 | - |
5-hydroxytriptonide | in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | neither knockdown nor over-expression of DCTPP1 significantly modifies the fraction of anti-BrdU reactive cells | additional information |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
5-iodo-dCTP | in 50 mM Tris, pH 8.0, 100 mM KCl, 5 mM MgCl2, 100 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C | Homo sapiens |