Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as His-tagged wild type protein and His-tagged variants | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D106A | variant displays reduced activity with a turnover value of 35% compared to the wild type counterpart | Homo sapiens |
D179A | variant is inactive | Homo sapiens |
D28A | variant is inactive | Homo sapiens |
H107N | variant displays reduced activity with a turnover value of 4.4% compared to the wild type counterpart, Km value increases 7fold | Homo sapiens |
H108N | variant displays reduced activity with a turnover value of 32% compared to the wild type counterpart | Homo sapiens |
N24H | variant is inactive | Homo sapiens |
R128H | enhanced kcat value (146%) is obtained with the mutant protein compared to the wild type counterpart, Km value increases 21fold | Homo sapiens |
R348A | variant is inactive | Homo sapiens |
General Stability | Organism |
---|---|
Mg2+ stabilizes h-prune against inactivation during storage | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(phosphate)25 | - |
Homo sapiens | |
(phosphate)45 | - |
Homo sapiens | |
(phosphate)65 | - |
Homo sapiens | |
diphosphate | potential physiological regulator | Homo sapiens | |
dipyridamole | known inhibitor of phosphodiesterase, no effect on the triphosphate hydrolyzing activity of h-prune | Homo sapiens | |
long-chain polyphosphate | potential physiological regulator, inhibits h-prune-catalyzed hydrolysis of triphosphate | Homo sapiens | |
additional information | nucleoside triphosphates, diadenosine hexaphosphate, cAMP and dipyridamole do not affect the activity | Homo sapiens | |
nm23-H1 | metastasis suppressor protein, the exopolyphosphatase activity is suppressed | Homo sapiens | |
ZnCl2 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
(phosphate)65 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.0012 | - |
(phosphate)3 | D106A mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.0022 | - |
(phosphate)3 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.0022 | - |
(phosphate)45 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.0026 | - |
(phosphate)3 | H107N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.0066 | - |
(phosphate)3 | 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.0069 | - |
(phosphate)25 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.015 | - |
(phosphate)3 | H108N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.019 | - |
(phosphate)4 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.028 | - |
adenosine 5'-tetraphosphate | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.037 | - |
adenosine 5'-tetraphosphate | 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.041 | - |
(phosphate)4 | 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.041 | - |
guanosine 5'-tetraphosphate | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.046 | - |
(phosphate)3 | R128H mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.099 | - |
guanosine 5'-tetraphosphate | 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | reaction requires a divalent metal cofactor | Homo sapiens | |
Mg2+ | reaction requires a divalent metal cofactor, bound substrate enhances enzyme affinity for the metal ion | Homo sapiens | |
Mn2+ | reaction requires a divalent metal cofactor, Mn2+ confers 50% activity compared to Mg2+ in P3 and P4 hydrolysis | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
estimated from the amino acid composition | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)n + H2O | Homo sapiens | h-prune efficiently hydrolyzes short-chain polyphosphates | (phosphate)n-1 + phosphate | - |
? | |
additional information | Homo sapiens | h-prune is the missing exopolyphosphatase in animals and support the hypothesis that the metastatic effects of h-prune are modulated by inorganic polyphosphates, which are increasingly recognized as critical regulators in cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by metal chelate chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
h-prune efficiently hydrolyzes short-chain polyphosphates, including inorganic tripoly- and tetrapolyphosphates and nucleoside 5'-tetraphosphates, long-chain inorganic polyphosphates (more than 25 phosphate residues) are converted more slowly | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(phosphate)25 + H2O | - |
Homo sapiens | (phosphate)24 + phosphate | - |
? | |
(phosphate)4 + H2O | - |
Homo sapiens | (phosphate)3 + phosphate | - |
? | |
(phosphate)45 + H2O | - |
Homo sapiens | (phosphate)44 + phosphate | - |
? | |
(phosphate)65 + H2O | - |
Homo sapiens | (phosphate)64 + phosphate | - |
? | |
(phosphate)n + H2O | h-prune efficiently hydrolyzes short-chain polyphosphates | Homo sapiens | (phosphate)n-1 + phosphate | - |
? | |
adenosine 5'-tetraphosphate + H2O | - |
Homo sapiens | ATP + phosphate | - |
? | |
guanosine 5'-tetraphosphate + H2O | - |
Homo sapiens | GTP + phosphate | - |
? | |
additional information | h-prune is the missing exopolyphosphatase in animals and support the hypothesis that the metastatic effects of h-prune are modulated by inorganic polyphosphates, which are increasingly recognized as critical regulators in cells | Homo sapiens | ? | - |
? | |
triphosphate + H2O | - |
Homo sapiens | diphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
exopolyphosphatase | - |
Homo sapiens |
h-prune | - |
Homo sapiens |
PPX | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
incubation of h-prune 1-100 microM for 5 h in the presence of 0.1 M Tris-HCl, pH 7.2, and 0.05 mM EGTA inactivates the enzyme 2-4fold, no inactivation is evident in the presence of 1 mM Mg2+ | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
(phosphate)65 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.16 | - |
(phosphate)25 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.22 | - |
(phosphate)45 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
0.57 | - |
(phosphate)3 | H107N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
3.4 | - |
(phosphate)4 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
4.2 | - |
(phosphate)3 | H108N mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
4.6 | - |
(phosphate)3 | D106A mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
7.1 | - |
(phosphate)4 | 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
13 | - |
(phosphate)3 | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
19 | - |
(phosphate)3 | R128H mutant, 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
24 | - |
guanosine 5'-tetraphosphate | 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
27 | - |
guanosine 5'-tetraphosphate | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
31 | - |
adenosine 5'-tetraphosphate | 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
35 | - |
(phosphate)3 | 2 mM Co2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens | |
39 | - |
adenosine 5'-tetraphosphate | 2 mM Mg2+ as cofactor, 0.1 M Tris-HCl, pH 7.2, 0.05 mM EGTA | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
2 mM Mg2+, 60 microM (phosphate)25, 10 mM triphosphate, IC50 is above 0.01 mM | Homo sapiens | nm23-H1 | |
0.0019 | - |
2 mM Mg2+, 10 mM triphosphate | Homo sapiens | nm23-H1 | |
0.0072 | - |
2 mM Mg2+, 10 mM triphosphate | Homo sapiens | nm23-H1 | |
0.032 | - |
2 mM Mg2+, hydrolysis of triphospate is inhibited | Homo sapiens | ZnCl2 | |
0.055 | - |
2 mM Mg2+, 10 mM triphosphate | Homo sapiens | (phosphate)45 | |
0.064 | - |
c2 mM Mg2+, 10 mM triphosphate | Homo sapiens | (phosphate)65 | |
0.105 | - |
c2 mM Mg2+, 100 mM triphosphate | Homo sapiens | (phosphate)65 | |
0.12 | - |
2 mM Mg2+, 100 mM triphosphate | Homo sapiens | (phosphate)45 | |
0.163 | - |
2 mM Mg2+, 10 mM triphosphate | Homo sapiens | (phosphate)25 | |
0.33 | - |
2 mM Mg2+, 100 mM triphosphate | Homo sapiens | (phosphate)25 | |
1.2 | - |
2 mM Mg2+, 10 mM triphosphate | Homo sapiens | diphosphate | |
5.7 | - |
2 mM Mg2+, 100 mM triphosphate | Homo sapiens | diphosphate |